Sphingoid bases of dietary ceramide 2-aminoethylphosphonate, a marine sphingolipid, absorb into lymph in rats

Nami Tomonaga, Tsuyoshi Tsuduki, Yuki Manabe, Tatsuya Sugawara

Research output: Contribution to journalReview articlepeer-review

8 Citations (Scopus)

Abstract

Various functions of dietary sphingolipids have been reported; however, little is known about marine sphingolipids. Ceramide 2-aminoethylphosphonate (CAEP), an abundant sphingolipid in marine mollusks, frequently has a unique triene type of sphingoid base [2-amino-9-methyl-4,8,10-octadecatriene-1,3-diol (d19:3)]. We previously reported that dietary CAEP prepared from the skin of squid was digested in the intestinal mucosa of mice via ceramides to yield free sphingoid bases. How dietary CAEP is then used in the body remains unclear. Here, we investigated the absorption of dietary CAEP using a lipid absorption assay on the lymph collected from rats with thoracic duct cannulation. Our results reveal that sphingoid bases derived from CAEP, including d16:1, d18:1, and d19:3, were detected in the lymph after administration of CAEP. Lymphatic recovery of d19:3 was lower than that of other sphingoid bases. A large fraction of the absorbed sphingoid bases was present as complex sphingolipids, whereas a smaller portion was present in the free form. Fatty acids in ceramide moieties found in the lymph were partially different from dietary CAEP, which indicates that sphingoid bases derived from CAEP could be, at least in part, resynthesized into complex sphingolipids. Future studies should elucidate the metabolism of sphingoid bases derived from CAEP.—Tomonaga, N., T. Tsuduki, Y. Manabe, and T. Sugawara. Sphingoid bases of dietary ceramide 2-ami-noethylphosphonate, a marine sphingolipid, absorb into lymph in rats.

Original languageEnglish
Pages (from-to)333-340
Number of pages8
JournalJournal of lipid research
Volume60
Issue number2
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • Dietary sphingolipids
  • Intestinal digestion
  • Lymph cannulation
  • Sphingolipid metabolism

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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