Sphingoid bases of dietary ceramide 2-aminoethylphosphonate, a marine sphingolipid, absorb into lymph in rats

Nami Tomonaga, Tsuyoshi Tsuduki, Yuki Manabe, Tatsuya Sugawara

    Research output: Contribution to journalReview articlepeer-review

    15 Citations (Scopus)

    Abstract

    Various functions of dietary sphingolipids have been reported; however, little is known about marine sphingolipids. Ceramide 2-aminoethylphosphonate (CAEP), an abundant sphingolipid in marine mollusks, frequently has a unique triene type of sphingoid base [2-amino-9-methyl-4,8,10-octadecatriene-1,3-diol (d19:3)]. We previously reported that dietary CAEP prepared from the skin of squid was digested in the intestinal mucosa of mice via ceramides to yield free sphingoid bases. How dietary CAEP is then used in the body remains unclear. Here, we investigated the absorption of dietary CAEP using a lipid absorption assay on the lymph collected from rats with thoracic duct cannulation. Our results reveal that sphingoid bases derived from CAEP, including d16:1, d18:1, and d19:3, were detected in the lymph after administration of CAEP. Lymphatic recovery of d19:3 was lower than that of other sphingoid bases. A large fraction of the absorbed sphingoid bases was present as complex sphingolipids, whereas a smaller portion was present in the free form. Fatty acids in ceramide moieties found in the lymph were partially different from dietary CAEP, which indicates that sphingoid bases derived from CAEP could be, at least in part, resynthesized into complex sphingolipids. Future studies should elucidate the metabolism of sphingoid bases derived from CAEP.—Tomonaga, N., T. Tsuduki, Y. Manabe, and T. Sugawara. Sphingoid bases of dietary ceramide 2-ami-noethylphosphonate, a marine sphingolipid, absorb into lymph in rats.

    Original languageEnglish
    Pages (from-to)333-340
    Number of pages8
    JournalJournal of lipid research
    Volume60
    Issue number2
    DOIs
    Publication statusPublished - 2019

    Keywords

    • Dietary sphingolipids
    • Intestinal digestion
    • Lymph cannulation
    • Sphingolipid metabolism

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Cell Biology

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