Spermatogenic Disturbance Induced in Mice by Combined Local Injection of Monoclonal Antibodies to Sertoli Cell and to Basal Lamina of Seminiferous Tubule

RYO ICHINOHASAMA, KATSUIKU HIROKAWA, SHIGERU HATAKEYAMA

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17 Citations (Scopus)

Abstract

ABSTRACT: Two kinds of hybridoma clones, one producing monoclonal antibodies against Sertoli cell (TM‐1) and the other the basal lamina of the seminiferous tubule (TM‐2), were raised by fusion between P3X63Ag8‐653 mouse myeloma cells and spleen cells of BALB/c mice (H‐2d) immunized with testicular homogenate of the same inbred mice. Immunohistochemically, TM‐1 reacted specifically with cytoplasmic component of Sertoli cell and TM‐2 with basal lamina of the seminiferous tubule. Using these monoclonal antibodies, spermatogenic disturbance was induced experimentally in BDF1(H‐2b/d) by intratesticular injection of a set of these two antibodies. Single injection of either TM‐1 or TM‐2 failed to induce the lesion. This fact indicated that TM‐1 antibody could reach the Sertoli cell to impair its function, which was otherwise inaccessible without coincidental action of TM‐2 antibody. TM‐2 antibody appeared to alter the permeability of the basal lamina of the tubule and lower its barrier effect. 1986 Munksgaard

Original languageEnglish
Pages (from-to)158-165
Number of pages8
JournalAmerican Journal of Reproductive Immunology and Microbiology
Volume10
Issue number4
DOIs
Publication statusPublished - 1986 Apr
Externally publishedYes

Keywords

  • Sertoli cell
  • Spermatogenic disturbance
  • basal lamina of seminiferous tubule
  • monoclonal antibody
  • mouse

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynaecology

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