Spectroscopic analysis of DNA duplexes including T-T mismatches.

Hiroshi Yamaguchi, Shuji Oda, Yoshinori Kondo, Akira Ono, Yoshiyuki Tanaka

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The DNA duplex with tandem T x T mismatches has been studied by NMR spectroscopy. For this study, we synthesized decamer duplex with two successive T x T mismatches, TT10:d(CGCGTTGTCC) x d(GGACTTCGCG). From two-dimensional (2D) 1H-1H NOESY spectrum, we were able to trace sequential NOE walks between base protons and anomeric protons (H1'), and assigned all the base protons and anomeric protons. These results indicate that the overall structure of the duplex is a right-handed duplex, with two successive T x T mismatches stacked in the helix.

Original languageEnglish
Pages (from-to)113-114
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number48
Publication statusPublished - 2004

ASJC Scopus subject areas

  • Medicine(all)

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