Spectroscopic analysis of AgI-DNA complex.

Shuji Oda, Tomoyuki Komuro, Yoshinori Kondo, Akira Ono, Yoshiyuki Tanaka

Research output: Contribution to journalArticlepeer-review

Abstract

It has recently been reported that C-C mismatches were able to recognize Ag(I) specifically, and form Ag(I)-mediated cytosine-cytosine base pairs (C-Ag(I)-C pair). In this study, we explored DNA sequences, which are appropriate for structural studies of this new type of metal-mediated base pairs. For this experiments, we synthesized four kinds of DNA duplexes including a single C-C mismatch. By using UV spectra, we performed titration experiments of DNA duplexes with AgNO(3). Within the sequences we tested, it was found that the DNA duplex: 5'-CGCGCGTCC-3' x 5'-GGACCCGCG-3' showed 1:1 complexation of this DNA duplex and Ag(I). In addition, a DNA duplex: 5'-TAAATCTAATA-3' x 5'-TATTACATTTA-3' also exhibited a similar character. Therefore, these two DNA duplexes are interesting candidates for structural analyses.

Original languageEnglish
Pages (from-to)199-200
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number50
DOIs
Publication statusPublished - 2006

ASJC Scopus subject areas

  • Medicine(all)

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