Spectral Assignments and Reference Data Complete¹H and¹³C resonance assignments of glycosidic conjugates of non-amidated and amidated bile acids

Complete ¹H and ¹³C resonance assignments on the basis of ID and 2D NMR were made for seven naturally occurring variants of the 3α-, 6α- and 7β-O-β-D-glycosidic conjugates (glucosides, N-acetylglucosaminides and glucuronides) of non-amidated and glycine- and taurine-amidated bile acids related to lithocholic, hyodeoxycholic, chenodeoxycholic, ursodeoxycolic, deoxycholic and cholic acids. The ¹H and ¹³C NMR signals serving to characterize each of the glycosidic conjugates of bile acids and the effects of the O-β-D-glucosidation, O-β-D-N-acetylglucosaminidation and O-β-D-glucuronidation at the 3-, 6- or 7-position in the 5β-steroid nucleus on the ¹H and ¹³C resonances were clarified and used in the structural elucidation of these bile acid glycosides.