Specificity of 4‴-acetylation by an aminoglycoside-modifying enzyme in arbekacin-resistant strains of methicillin-resistant Staphylococcus aureus

Shigeru Fujimuba, Yutaka Tokue, Hiroshi Takahashi, Toshihiro Nukiwa, Akira Watanabe

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Four arbekacin (ABK)-resistant clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were studied regarding their mechanism of aminoglycoside-resistance. Metabolites of amikacin (AMK) and gentamicin(GM) were obtained by reaction with excess amounts of crude enzyme preparation extracted from the ABK-resistant MRSA strains. Then the metabolites were then isolated and analyzed by means of 1H, 13C-nuclear magnetic resonance. The AMK-modification was 4‴-N-acetylation. However, the site of GM-acetylation may be 6′-N-position by these strains.

    Original languageEnglish
    Pages (from-to)67-70
    Number of pages4
    JournalTohoku Journal of Experimental Medicine
    Volume186
    Issue number1
    Publication statusPublished - 1998 Sep 1

    Keywords

    • Amikacin
    • Arbekacin-resistance
    • MRSA
    • NMR

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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