Specific miRNA Stabilization by Gld2-Catalyzed Monoadenylation

Andrea D'Ambrogio, Weifeng Gu, Tsuyoshi Udagawa, Craig C. Mello, Joel D. Richter

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small, noncoding RNAs that inhibit translation and promote mRNA decay. The levels of mature miRNAs are the result of different rates of transcription, processing, and turnover. The noncanonical polymerase Gld2 has been implicated in the stabilization of miR-122, possibly through catalyzing 3@ monoadenylation; however, there is little evidence that this relationship is one of cause and effect. Here, we biochemically characterize Gld2@s involvement in miRNA monoadenylation and its effect on miRNA stability. We find that Gld2 directly monoadenylates and stabilizes specific miRNA populations in human fibroblasts and that sensitivity to monoadenylation-induced stability depends on nucleotides in the miRNA 3@ end. These results establish a mechanism of miRNA stability and resulting posttranscriptional gene regulation.

Original languageEnglish
Pages (from-to)1537-1545
Number of pages9
JournalCell Reports
Volume2
Issue number6
DOIs
Publication statusPublished - 2012 Dec 27
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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