TY - JOUR
T1 - Specific Inhibitors of Vacuolar Type H+-ATPases Induce Apoptotic Cell Death
AU - Nishihara, Tatsuji
AU - Akifusa, Sumio
AU - Koseki, Takeyoshi
AU - Kato, Satsuki
AU - Muro, Miyuki
AU - Hanada, Nobuhiro
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1995
Y1 - 1995
N2 - Concanamycin A and bafilomycin A1 are known as strong inhibitors of the vacuolar type H+-ATPases in vitro. These inhibitors exhibited cytotoxic effects on twelve cell lines in cell viability assay. On the other hand, the F1F0-type H+-ATPase inhibitor oligomycin and the E1E2-type H+-ATPase inhibitor vanadate showed no cytotoxic effect. We show here that concanamycin A and bafilomycin A1 induce a significant increase in the proportion of fragmented DNA in agarose gel electrophoresis. Flow cytometric cell cycle analysis of WEHI 231 cells stimulated with concanamycin A revealed the increased percentage of apoptotic cells with hypodiploid DNA. These findings indicate that cell death induced by specific inhibitors of vacuolar type H+-ATPases occurs through apoptosis.
AB - Concanamycin A and bafilomycin A1 are known as strong inhibitors of the vacuolar type H+-ATPases in vitro. These inhibitors exhibited cytotoxic effects on twelve cell lines in cell viability assay. On the other hand, the F1F0-type H+-ATPase inhibitor oligomycin and the E1E2-type H+-ATPase inhibitor vanadate showed no cytotoxic effect. We show here that concanamycin A and bafilomycin A1 induce a significant increase in the proportion of fragmented DNA in agarose gel electrophoresis. Flow cytometric cell cycle analysis of WEHI 231 cells stimulated with concanamycin A revealed the increased percentage of apoptotic cells with hypodiploid DNA. These findings indicate that cell death induced by specific inhibitors of vacuolar type H+-ATPases occurs through apoptosis.
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U2 - 10.1006/bbrc.1995.1964
DO - 10.1006/bbrc.1995.1964
M3 - Article
C2 - 7612014
AN - SCOPUS:0029081810
VL - 212
SP - 255
EP - 262
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -