Concanamycin A and bafilomycin A1 are known as strong inhibitors of the vacuolar type H+-ATPases in vitro. These inhibitors exhibited cytotoxic effects on twelve cell lines in cell viability assay. On the other hand, the F1F0-type H+-ATPase inhibitor oligomycin and the E1E2-type H+-ATPase inhibitor vanadate showed no cytotoxic effect. We show here that concanamycin A and bafilomycin A1 induce a significant increase in the proportion of fragmented DNA in agarose gel electrophoresis. Flow cytometric cell cycle analysis of WEHI 231 cells stimulated with concanamycin A revealed the increased percentage of apoptotic cells with hypodiploid DNA. These findings indicate that cell death induced by specific inhibitors of vacuolar type H+-ATPases occurs through apoptosis.
|Number of pages||8|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 1995|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology