Specific induction of adhesion molecules in human vascular endothelial cells by rat experimental pancreatitis-associated ascitic fluids

Atsushi Masamune, Tooru Shimosegawa, Kenji Kimura, Motokazu Fujita, Akihiko Sato, Masaru Koizumi, Takayoshi Toyota

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

The molecular mechanisms that link acute pancreatitis (AP) and multiple organ failure remain unknown. To clarify the role of endothelial activation, we examined the effects of ascitic fluids from rats with experimental pancreatitis on the expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs). Necrotizing hemorrhagic pancreatitis was induced with sodium taurocholate. Six and 24 h later, peritoneal exudates were collected, centrifuged and HUVECs were treated with the supernatants. The expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was quantified by enzyme-linked immunosorbent assay. Induction of mRNA was assessed by reverse-transcriptase polymerase chain reaction. The activation of transcription factors was examined by electrophoretic mobility shift assay. The expression of ICAM-1 in the tissues was examined immunohistochemically. ICAM-1 and VCAM-1, but not E- selectin expression was upregulated with comparable mRNA induction. Nuclear factor κB was activated, while activator protein-1 binding activity was not altered. Immunohistochemically, enhanced ICAM-1 expression was observed in the pancreas and lung, but not in the liver. Ascitic fluids may contain soluble factors responsible for the transcriptional activation of endothelial adhesion molecules, and ICAM-1 may play roles in the pathogenesis of complicated AP.

Original languageEnglish
Pages (from-to)141-150
Number of pages10
JournalPancreas
Volume18
Issue number2
DOIs
Publication statusPublished - 1999 Mar

Keywords

  • Acute pancreatitis
  • Endothelial cell
  • Experimental pancreatitis
  • Intercellular adhesion molecule-1
  • Nuclear factor-κB

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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