Specific inducibility of globin mRNA in Friend leukemia cells in low serum concentration

K. Nose, H. Okamoto

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Friend leukemia cells were adapted to grow in a medium containing 0.5% serum by gradually decreasing concentration of the serum from 15%. Upon treatment of the cells with dimethyl sulfoxide, appearance of benzidine-positive cells was suppressed in the cells cultivated in a 0.5% serum medium, whereas globin mRNA sequences, as determined by hybridization of cytoplasmic RNA with globin cDNA, increased to the same order as in the cells grown in a medium containing 15% serum. A small but significant amount of globin was synthesized in cells treated with dimethyl sulfoxide in 0.5% serum medium, as determined by dodecyl sulfate-polyacrylamide gel electrophoresis of 3H-labeled cellular proteins.

Original languageEnglish
Pages (from-to)80-83
Number of pages4
JournalGann, The Japanese Journal of Cancer Research
Volume71
Issue number1
Publication statusPublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research

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