Abstract
Friend leukemia cells were adapted to grow in a medium containing 0.5% serum by gradually decreasing concentration of the serum from 15%. Upon treatment of the cells with dimethyl sulfoxide, appearance of benzidine-positive cells was suppressed in the cells cultivated in a 0.5% serum medium, whereas globin mRNA sequences, as determined by hybridization of cytoplasmic RNA with globin cDNA, increased to the same order as in the cells grown in a medium containing 15% serum. A small but significant amount of globin was synthesized in cells treated with dimethyl sulfoxide in 0.5% serum medium, as determined by dodecyl sulfate-polyacrylamide gel electrophoresis of 3H-labeled cellular proteins.
Original language | English |
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Pages (from-to) | 80-83 |
Number of pages | 4 |
Journal | Gann, The Japanese Journal of Cancer Research |
Volume | 71 |
Issue number | 1 |
Publication status | Published - 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Cancer Research