Specific direct radioimmunoassay of angiotensin II (AT II) in human plasma and the effect of angiotensin converting enzyme (ACE) inhibitor

Fumiaki Abe, Ken Omata, Masaaki Yamada, Kazuo Tsunoda, Tatsuyuki Sato, Tetsu Shimizu, Sadayoshi Ito, Keishi Abe, Megumi Nakazima, Taeko Morimoto, Naoki Takanashi

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Abstract

Specific direct radioimmunoassay of angiotensin II (AT II) in human plasma was developed to evaluate the dynamics of endogenous AT II in various types of hypertension. Detection limit of this method was less than 2.3 pg/ml, and normal value is less than 25 pg/ml. Cross reactivity of antibody with AT I and III was 0.037% and 21%, respectively. There were good correlation between the value measured by direct method, and that of extraction method (r=0.96, P<0.01) and plasma renin activity (r=0.80, P<0.01). By oral administration of ACE inhibitor (captopril 50 mg), AT II levels were suppressed to 10 pg/ml or less in most patients with essential hypertension, renal parenchymal hypertension and renovascular hypertension up to 2 h. However, AT II levels in patients treated with ACE inhibitors chronically were not different from the AT II levels in patients without ACE inhibitor. In primary aldosteronism AT II was extremely low levels. AT II markedly increased by the stimulation test using furosemide (1 mg/kg i.v.). These results suggest that this method may be useful to clarify the pathophysiology of hypertension and the escape of the inhibition by ACE inhibitor. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalImmunopharmacology
Volume44
Issue number1-2
DOIs
Publication statusPublished - 1999 Oct 15

Keywords

  • Angiotensin II
  • Angiotensin converting enzyme (ACE) inhibitor
  • Hypertension
  • Renin

ASJC Scopus subject areas

  • Pharmacology

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    Abe, F., Omata, K., Yamada, M., Tsunoda, K., Sato, T., Shimizu, T., Ito, S., Abe, K., Nakazima, M., Morimoto, T., & Takanashi, N. (1999). Specific direct radioimmunoassay of angiotensin II (AT II) in human plasma and the effect of angiotensin converting enzyme (ACE) inhibitor. Immunopharmacology, 44(1-2), 199-204. https://doi.org/10.1016/S0162-3109(99)00090-9