Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling

Minoru Sato; Toshiki Nakano; Masaaki Takeuchi; Tsutomu Kumagai; Nobuhiro Kanno; Eizou Nagahisa; Yoshikazu Sato

doi:10.1271/bbb.59.1208

Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling

Minoru Sato, Toshiki Nakano, Masaaki Takeuchi, Tsutomu Kumagai, Nobuhiro Kanno, Eizou Nagahisa, Yoshikazu Sato

AGR - Biological Resource Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.

Original language	English
Pages (from-to)	1208-1210
Number of pages	3
Journal	Bioscience, Biotechnology, and Biochemistry
Volume	59
Issue number	7
DOIs	https://doi.org/10.1271/bbb.59.1208
Publication status	Published - 1995

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.59.1208

Cite this

Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling. / Sato, Minoru; Nakano, Toshiki; Takeuchi, Masaaki; Kumagai, Tsutomu; Kanno, Nobuhiro; Nagahisa, Eizou; Sato, Yoshikazu.

In: Bioscience, Biotechnology, and Biochemistry, Vol. 59, No. 7, 1995, p. 1208-1210.

Research output: Contribution to journal › Article › peer-review

@article{abc471136b3e42d4aeda77e4d073f0e4,

title = "Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling",

abstract = "A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.",

author = "Minoru Sato and Toshiki Nakano and Masaaki Takeuchi and Tsutomu Kumagai and Nobuhiro Kanno and Eizou Nagahisa and Yoshikazu Sato",

year = "1995",

doi = "10.1271/bbb.59.1208",

language = "English",

volume = "59",

pages = "1208--1210",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "7",

}

TY - JOUR

T1 - Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling

AU - Sato, Minoru

AU - Nakano, Toshiki

AU - Takeuchi, Masaaki

AU - Kumagai, Tsutomu

AU - Kanno, Nobuhiro

AU - Nagahisa, Eizou

AU - Sato, Yoshikazu

PY - 1995

Y1 - 1995

N2 - A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.

AB - A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.

UR - http://www.scopus.com/inward/record.url?scp=0029340110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029340110&partnerID=8YFLogxK

U2 - 10.1271/bbb.59.1208

DO - 10.1271/bbb.59.1208

M3 - Article

C2 - 7670179

AN - SCOPUS:0029340110

VL - 59

SP - 1208

EP - 1210

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 7

ER -

Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this