Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling

Minoru Sato, Toshiki Nakano, Masaaki Takeuchi, Tsutomu Kumagai, Nobuhiro Kanno, Eizou Nagahisa, Yoshikazu Sato

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.

Original languageEnglish
Pages (from-to)1208-1210
Number of pages3
JournalBioscience, Biotechnology, and Biochemistry
Volume59
Issue number7
DOIs
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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