Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling

Minoru Sato; Toshiki Nakano; Masaaki Takeuchi; Tsutomu Kumagai; Nobuhiro Kanno; Eizou Nagahisa; Yoshikazu Sato

doi:10.1271/bbb.59.1208

Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling

Minoru Sato, Toshiki Nakano, Masaaki Takeuchi, Tsutomu Kumagai, Nobuhiro Kanno, Eizou Nagahisa, Yoshikazu Sato

AGR - Biological Resource Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.

Original language	English
Pages (from-to)	1208-1210
Number of pages	3
Journal	Bioscience, Biotechnology, and Biochemistry
Volume	59
Issue number	7
DOIs	https://doi.org/10.1271/bbb.59.1208
Publication status	Published - 1995

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling. / Sato, Minoru; Nakano, Toshiki; Takeuchi, Masaaki; Kumagai, Tsutomu; Kanno, Nobuhiro; Nagahisa, Eizou; Sato, Yoshikazu.

In: Bioscience, Biotechnology, and Biochemistry, Vol. 59, No. 7, 1995, p. 1208-1210.

Research output: Contribution to journal › Article › peer-review

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AU - Sato, Minoru

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AU - Takeuchi, Masaaki

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AU - Kanno, Nobuhiro

AU - Nagahisa, Eizou

AU - Sato, Yoshikazu

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Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling

Abstract

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