Specific cytolysis of HIV-infected cells by lymphocytes armed with bifunctional antibodies

Hidechika Okada, Hiroshi Momota, Takachika Azuma, Toshio Hattori, Noriko Okada

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The Fab′ fragment of a monoclonal antibody (mAb) directed to CD3 (a portion of the T cell receptor) and the Fab′ or F(ab′)2 fragment of an mAb to HIV were combined to generate bifunctional antibody (BFA) consisting of antiCD3-Fab′ conjugated with anti-HIV-Fab′ (Fab′/Fab′) or antiCD3-Fab′ conjugated with anti-HIV-F(ab′)2 (Fab′/F(ab′)2), respectively. In the presence of these BFA, HIV-infected target cells were cytolysed by peripheral blood lymphocytes. Treatment of lymphocytes with Fab′/F(ab′)2 type BFA rendered the lymphocytes significantly cytotoxic to HIV-infected target cells. Since BFA-armed lymphocytes can react on HIV-infected cells regardless of histocompatibility, lymphocytes from healthy donors could be armed with BFA for treatment of HIV-infected patients including those who do not exhibit histocompatibility with the lymphocyte donor.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalImmunology Letters
Volume31
Issue number3
DOIs
Publication statusPublished - 1992 Feb 15

Keywords

  • Bifunctional antibody
  • F(ab′)
  • Human immunodeficiency virus
  • Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Specific cytolysis of HIV-infected cells by lymphocytes armed with bifunctional antibodies'. Together they form a unique fingerprint.

Cite this