Specific and efficient targeting of cyanobacterial bicarbonate transporters to the inner envelope membrane of chloroplasts in arabidopsis

Susumu Uehara, Fumi Adachi, Yasuko Ito-Inaba, Takehito Inaba

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Installation of cyanobacterial bicarbonate transporters to the inner envelope membrane (IEM) of chloroplasts in C3 plants has been thought to improve photosynthetic performance. However, the method to deliver cyanobacterial bicarbonate transporters to the chloroplast IEM remains to be established. In this study, we provide evidence that the cyanobacterial bicarbonate transporters, BicA and SbtA, can be specifically installed into the chloroplast IEM using the chloroplast IEM targeting signal in conjunction with the transit peptide. We fused the transit peptide and the mature portion of Cor413im1, whose targeting mechanism to the IEM has been characterized in detail, to either BicA or SbtA isolated from Synechocystis sp. PCC6803. Among the seven chimeric constructs tested, we confirmed that four chimeric bicarbonate transporters, designated as BicAI, BicAII, SbtAII, and SbtAIII, were expressed in Arabidopsis. Furthermore, these chimeric transporters were specifically targeted to the chloroplast IEM. They were also resistant to alkaline extraction but can be solubilized by Triton X-100, indicating that they are integral membrane proteins in the chloroplast IEM. One of the transporters, BicA, could reside in the chloroplast IEM even after removal of the IEM targeting signal. Taken together, our results indicate that the addition of IEM targeting signal, as well as the transit peptide, to bicarbonate transporters allows us to efficiently target nuclear-encoded chimeric bicarbonate transporters to the chloroplast IEM.

Original languageEnglish
Article number16
JournalFrontiers in Plant Science
Volume7
Issue numberFEB2016
DOIs
Publication statusPublished - 2016 Feb 2
Externally publishedYes

Keywords

  • Arabidopsis
  • Bicarbonate transporter
  • Chloroplast
  • Cyanobacteria
  • Protein targeting

ASJC Scopus subject areas

  • Plant Science

Fingerprint

Dive into the research topics of 'Specific and efficient targeting of cyanobacterial bicarbonate transporters to the inner envelope membrane of chloroplasts in arabidopsis'. Together they form a unique fingerprint.

Cite this