Species differences of macrophage very low-density-lipoprotein (VLDL) receptor protein expression

Sadao Takahashi, Takashi Ito, Yasuo Zenimaru, Jinya Suzuki, Isamu Miyamori, Masao Takahashi, Masafumi Takahashi, Takafumi Ishida, Tatsuro Ishida, Ken ichi Hirata, Tokuo T. Yamamoto, Tadao Iwasaki, Hiroaki Hattori, Masashi Shiomi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Triglyceride-rich lipoproteins (TGRLs) and low-density-lipoprotein (LDL) cholesterol are independent risk factors for coronary artery disease. We have previously proposed that the very low-density-lipoprotein (VLDL) receptor is one of the receptors required for foam cell formation by TGRLs in human macrophages. However, the VLDL receptor proteins have not been detected in atherosclerotic lesions of several animal models. Here we showed no VLDL receptor protein was detected in mouse macrophage cell lines (Raw264.7 and J774.2) or in mouse peritoneal macrophages in vitro. Furthermore, no VLDL receptor protein was detected in macrophages in atherosclerotic lesions of chow-fed apolipoprotein E-deficient or cholesterol-fed LDL receptor-deficient mice in vivo. In contrast, macrophage VLDL receptor protein was clearly detected in human macrophages in vitro and in atherosclerotic lesions in myocardial infarction-prone Watanabe-heritable hyperlipidemic (WHHLMI) rabbits in vivo. There are species differences in the localization of VLDL receptor protein in vitro and in vivo. Since VLDL receptor is expressed on macrophages in atheromatous plaques of both rabbit and human but not in mouse models, the mechanisms of atherogenesis and/or growth of atherosclerotic lesions in mouse models may be partly different from those of humans and rabbits.

Original languageEnglish
Pages (from-to)656-662
Number of pages7
JournalBiochemical and biophysical research communications
Issue number4
Publication statusPublished - 2011 Apr 22


  • Atherosclerosis
  • Foam cell formation
  • LDL receptor
  • Macrophages
  • Species differences
  • VLDL receptor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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