TY - JOUR
T1 - Spatiotemporally controlled contraction of micropatterned skeletal muscle cells on a hydrogel sheet
AU - Nagamine, Kuniaki
AU - Kawashima, Takeaki
AU - Sekine, Soichiro
AU - Ido, Yuichiro
AU - Kanzaki, Makoto
AU - Nishizawa, Matsuhiko
PY - 2011/2/7
Y1 - 2011/2/7
N2 - We have developed gel sheet-supported C2C12 myotube micropatterns and combined them with a microelectrode array chip to afford a skeletal muscle cell-based bioassay system. Myotube line patterns cultured on a glass substrate were transferred with 100% efficiency to the surface of fibrin gel sheets. The contractile behavior of each myotube line pattern on the gel was individually controlled by localized electrical stimulation using microelectrode arrays that had been previously modified with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT). We successfully demonstrated fluorescent imaging of the contraction-induced translocation of the glucose transporter, GLUT4, from intracellular vesicles to the plasma membrane of the myotubes. This device is applicable for the bioassay of contraction-induced metabolic alterations in a skeletal muscle cell.
AB - We have developed gel sheet-supported C2C12 myotube micropatterns and combined them with a microelectrode array chip to afford a skeletal muscle cell-based bioassay system. Myotube line patterns cultured on a glass substrate were transferred with 100% efficiency to the surface of fibrin gel sheets. The contractile behavior of each myotube line pattern on the gel was individually controlled by localized electrical stimulation using microelectrode arrays that had been previously modified with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT). We successfully demonstrated fluorescent imaging of the contraction-induced translocation of the glucose transporter, GLUT4, from intracellular vesicles to the plasma membrane of the myotubes. This device is applicable for the bioassay of contraction-induced metabolic alterations in a skeletal muscle cell.
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U2 - 10.1039/c0lc00364f
DO - 10.1039/c0lc00364f
M3 - Article
C2 - 21116545
AN - SCOPUS:78751474092
VL - 11
SP - 513
EP - 517
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 3
ER -