Spatiotemporally controlled contraction of micropatterned skeletal muscle cells on a hydrogel sheet

Kuniaki Nagamine, Takeaki Kawashima, Soichiro Sekine, Yuichiro Ido, Makoto Kanzaki, Matsuhiko Nishizawa

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

We have developed gel sheet-supported C2C12 myotube micropatterns and combined them with a microelectrode array chip to afford a skeletal muscle cell-based bioassay system. Myotube line patterns cultured on a glass substrate were transferred with 100% efficiency to the surface of fibrin gel sheets. The contractile behavior of each myotube line pattern on the gel was individually controlled by localized electrical stimulation using microelectrode arrays that had been previously modified with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT). We successfully demonstrated fluorescent imaging of the contraction-induced translocation of the glucose transporter, GLUT4, from intracellular vesicles to the plasma membrane of the myotubes. This device is applicable for the bioassay of contraction-induced metabolic alterations in a skeletal muscle cell.

Original languageEnglish
Pages (from-to)513-517
Number of pages5
JournalLab on a Chip
Volume11
Issue number3
DOIs
Publication statusPublished - 2011 Feb 7

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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