Spatiotemporal secretion of PEROXIDASE36 is required for seed coat mucilage extrusion in Arabidopsis

Tadashi Kunieda, Tomoo Shimada, Maki Kondo, Mikio Nishimura, Kazuhiko Nishitani, Ikuko Hara-Nishimura

    Research output: Contribution to journalArticlepeer-review

    49 Citations (Scopus)

    Abstract

    The epidermal cells of the Arabidopsis thaliana seed coat, which correspond to the second layer of the outer integument (oi2), contain large quantities of a pectic polysaccharide called mucilage within the apoplastic space beneath the outer periclinal cell wall. Immediately after seed imbibition, the mucilage is extruded and completely envelops the seed in a gel-like capsule. We found that a class III peroxidase family protein, PEROXIDASE36 (PER36), functions as a mucilage extrusion factor. Expression of PER36 occurred only in oi2 cells for a few days around the torpedo stage. A PER36-green fluorescent protein fusion was secreted into the outer cell wall in a polarized manner. per36 mutants were defective in mucilage extrusion after seed imbibition due to the failure of outer cell wall rupture, although the mutants exhibited normal monosaccharide composition of the mucilage. This abnormal phenotype of per36 was rescued by pectin solubilization, which promoted cell wall loosening. These results suggest that PER36 regulates the degradation of the outer cell wall. Taken together, this work indicates that polarized secretion of PER36 in a developmental stage-dependent manner plays a role in cell wall modification of oi2 cells.

    Original languageEnglish
    Pages (from-to)1355-1367
    Number of pages13
    JournalPlant Cell
    Volume25
    Issue number4
    DOIs
    Publication statusPublished - 2013 Apr

    ASJC Scopus subject areas

    • Plant Science
    • Cell Biology

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