Spatiotemporal expression of duplicate AGAMOUS orthologues during floral development in Phalaenopsis

In Ja Song, Toru Nakamura, Tatsuya Fukuda, Jun Yokoyama, Takuro Ito, Hiroaki Ichikawa, Yoh Horikawa, Toshiaki Kameya, Akira Kanno

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

The AGAMOUS (AG) family of MADS-box genes plays important roles in controlling the development of the reproductive organs of flowering plants. To understand the molecular mechanisms behind the floral development in the orchid, we isolated and characterized two AG-like genes from Phalaenopsis that we denoted PhalAG1 and PhalAG2. Phylogenetic analysis indicated that PhalAG1 and PhalAG2 fall into different phylogenetic positions in the AG gene family as they belong to the C- and D-lineages, respectively. Reverse transcription-polymerase chair reaction (RT-PCR) analyses showed that PhalAG1 and PhalAG2 transcripts were detected in flower buds but not in vegetative organs. Moreover, in situ hybridization experiments revealed that PhalAG1 and PhalAG2 hybridization signals were observed in the lip, column, and ovule during the floral development of Phalaenopsis, with little difference between the expression patterns of the two genes. These results suggest that both AG-like genes in Phalaenopsis act redundantly with each other in floral development.

Original languageEnglish
Pages (from-to)301-313
Number of pages13
JournalDevelopment Genes and Evolution
Volume216
Issue number6
DOIs
Publication statusPublished - 2006 Jun

Keywords

  • AGAMOUS-like gene
  • C-class gene
  • Column
  • D-class gene
  • Phalaenopsis

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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