Spatiogenetic characterization of s receptor kinase (Srk) alleles in naturalized populations of raphanus sativus l. var. raphanistroides on yakushima island

Kazuki Fukushima, Toko Kanomata, Aoi Kon, Hiromi Masuko-Suzuki, Kana Ito, Sadayoshi Ogata, Yoshinobu Takada, Yukihiro Komatsubara, Tsuyoshi Nakamura, Takumi Watanabe, Saori Koizumi, Hitoshi Sanuki, Jong In Park, Satoshi Niikura, Keita Suwabe, Sota Fujii, Kohji Murase, Seiji Takayama, Go Suzuki, Masao Watanabe

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In various coastal areas of Japan, naturalized radish populations are observed. Radish is a cruciferous plant and exhibits self-incompatibility, involving a system controlled by a single locus with multiple S alleles. Although the S allele diversity of radish cultivars and wild radishes has been characterized, the S allele distribution in naturalized populations has not yet been analyzed in relation to the positions of the plants in situ. Here, we show the S allele distribution in naturalized radish populations of Yakushima, a small island in the East China Sea, with positions of the plants. Radish plants were sampled in coastal areas in Yakushima, and their S alleles were detected and characterized. Most of the S alleles had been previously identified in radish cultivars. However, four novel S alleles, which may be unique to Yakushima, were also found. Moreover, seeds in siliques from plants growing in the study areas were sampled, and S allele determination in DNA extracted from these seeds suggested that the plants had exchanged their pollen among their close neighbors. There was also a problem in that the PCR amplification of some SRK alleles was difficult because of their sequence diversity in the naturalized populations, as occurs in cultivars. Our results suggest that the exchange of S alleles between cultivars and naturalized populations occurs and that S alleles in naturalized populations are highly diverse. The methodology established in our study should be applicable to other self-incompatible species to dissect the diversity of S allele distribution in naturalized populations.

Original languageEnglish
Pages (from-to)129-139
Number of pages11
JournalGenes and Genetic Systems
Volume96
Issue number3
DOIs
Publication statusPublished - 2021

Keywords

  • Naturalized population
  • Raphanus sativus L. var. raphanistroides
  • S allele
  • SRK gene
  • Self-incompatibility

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Spatiogenetic characterization of s receptor kinase (Srk) alleles in naturalized populations of raphanus sativus l. var. raphanistroides on yakushima island'. Together they form a unique fingerprint.

Cite this