TY - JOUR
T1 - Spatiogenetic characterization of s receptor kinase (Srk) alleles in naturalized populations of raphanus sativus l. var. raphanistroides on yakushima island
AU - Fukushima, Kazuki
AU - Kanomata, Toko
AU - Kon, Aoi
AU - Masuko-Suzuki, Hiromi
AU - Ito, Kana
AU - Ogata, Sadayoshi
AU - Takada, Yoshinobu
AU - Komatsubara, Yukihiro
AU - Nakamura, Tsuyoshi
AU - Watanabe, Takumi
AU - Koizumi, Saori
AU - Sanuki, Hitoshi
AU - Park, Jong In
AU - Niikura, Satoshi
AU - Suwabe, Keita
AU - Fujii, Sota
AU - Murase, Kohji
AU - Takayama, Seiji
AU - Suzuki, Go
AU - Watanabe, Masao
N1 - Funding Information:
The authors thank Makoto Endo (Takii & Co. Ltd.) for providing seeds of radish cultivars, and Keita Yamaki, Tai Takemoto and Misono Sasaki (Tohoku University) for technical assistance. This work was supported in part by MEXT KAKENHI (Grant Numbers 16H06467 to S. T.; 16H01467 and 18H04776 to S. F.; 16H06470, 16H06464 and 16K21727 to M. W.), JSPS KAKENHI (Grant Numbers 16H06380 to S. T.; 19K05963 to Y. T.; 20H02956 to K. S.; 18H02456, 19H02947 and 20K21417 to S. F.; 20K05982 to G. S.; 17H00821, 18KT0048 and 19K22342 to M. W.), JSPS Bilateral Programs (Grant Number 18032211-000481 to M. W.), JST PRESTO (Grant Number JPMJPR204A to K. M.), and by the NRF Basic Science Research Program (Grant Number NRF-2020R1I1A3073093), Republic of Korea to J.-I. P. This research was also supported by the Global Science Campus program of the Japan Science and Technology Agency to M. W.
Publisher Copyright:
© 2021 The Author(s).
PY - 2021
Y1 - 2021
N2 - In various coastal areas of Japan, naturalized radish populations are observed. Radish is a cruciferous plant and exhibits self-incompatibility, involving a system controlled by a single locus with multiple S alleles. Although the S allele diversity of radish cultivars and wild radishes has been characterized, the S allele distribution in naturalized populations has not yet been analyzed in relation to the positions of the plants in situ. Here, we show the S allele distribution in naturalized radish populations of Yakushima, a small island in the East China Sea, with positions of the plants. Radish plants were sampled in coastal areas in Yakushima, and their S alleles were detected and characterized. Most of the S alleles had been previously identified in radish cultivars. However, four novel S alleles, which may be unique to Yakushima, were also found. Moreover, seeds in siliques from plants growing in the study areas were sampled, and S allele determination in DNA extracted from these seeds suggested that the plants had exchanged their pollen among their close neighbors. There was also a problem in that the PCR amplification of some SRK alleles was difficult because of their sequence diversity in the naturalized populations, as occurs in cultivars. Our results suggest that the exchange of S alleles between cultivars and naturalized populations occurs and that S alleles in naturalized populations are highly diverse. The methodology established in our study should be applicable to other self-incompatible species to dissect the diversity of S allele distribution in naturalized populations.
AB - In various coastal areas of Japan, naturalized radish populations are observed. Radish is a cruciferous plant and exhibits self-incompatibility, involving a system controlled by a single locus with multiple S alleles. Although the S allele diversity of radish cultivars and wild radishes has been characterized, the S allele distribution in naturalized populations has not yet been analyzed in relation to the positions of the plants in situ. Here, we show the S allele distribution in naturalized radish populations of Yakushima, a small island in the East China Sea, with positions of the plants. Radish plants were sampled in coastal areas in Yakushima, and their S alleles were detected and characterized. Most of the S alleles had been previously identified in radish cultivars. However, four novel S alleles, which may be unique to Yakushima, were also found. Moreover, seeds in siliques from plants growing in the study areas were sampled, and S allele determination in DNA extracted from these seeds suggested that the plants had exchanged their pollen among their close neighbors. There was also a problem in that the PCR amplification of some SRK alleles was difficult because of their sequence diversity in the naturalized populations, as occurs in cultivars. Our results suggest that the exchange of S alleles between cultivars and naturalized populations occurs and that S alleles in naturalized populations are highly diverse. The methodology established in our study should be applicable to other self-incompatible species to dissect the diversity of S allele distribution in naturalized populations.
KW - Naturalized population
KW - Raphanus sativus L. var. raphanistroides
KW - S allele
KW - SRK gene
KW - Self-incompatibility
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U2 - 10.1266/GGS.20-00066
DO - 10.1266/GGS.20-00066
M3 - Article
C2 - 34148895
AN - SCOPUS:85118285793
VL - 96
SP - 129
EP - 139
JO - Genes and Genetic Systems
JF - Genes and Genetic Systems
SN - 1341-7568
IS - 3
ER -