Spatial and developmental profiling of miraculin accumulation in transgenic tomato fruits expressing the miraculin gene constitutively

You Wang Kim, Kato Kazuhisa, Hirai Tadayoshi, Hiwasa Tanase Kyoko, Ezura Hiroshi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We previously developed a transgenic tomato that expresses the miraculin gene using a constitutive promoter. In this study, we profiled the developmental and spatial accumulation of the miraculin protein and mRNA in transgenic tomato fruits. Miraculin mRNA expression was almost constant up to orange stage, and then the expression increased at red stage. The miraculin protein accumulated gradually during fruit development and reached its highest level at the overripe stage. At the red stage of fruit, miraculin protein was accumulated at the highest level in the exocarp, and similar in other fruit tissues: mesocarp, dissepiment, upper placenta, lower placenta and jelly. Moreover, the pattern of miraculin accumulation in fruit tissues was the same regardless of genetic background and position at which the miraculin gene was inserted in the genome. We also discuss suitable tomato types expressing miraculin for their commercial use.

Original languageEnglish
Pages (from-to)282-286
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number1
DOIs
Publication statusPublished - 2010 Jan 13
Externally publishedYes

Keywords

  • 35s promoter
  • Fruit tissue
  • Miraculin
  • Taste-modifying protein

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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