TY - JOUR
T1 - Soybean seed extracts preferentially express genomic loci of bradyrhizobium japonicum in the initial interaction with soybean, glycine max (L.) Merr
AU - Min, W. E.I.
AU - Yokoyama, Tadashi
AU - Minamisawa, Kiwamu
AU - Mitsui, Hisayuki
AU - Itakura, Manabu
AU - Kaneko, Takakazu
AU - Tabata, Satoshi
AU - Saeki, Kazuhiko
AU - Omori, Hirofumi
AU - Tajima, Shigeyuki
AU - Uchiumi, Toshiki
AU - Mikiko, A. B.E.
AU - Ohwada, Takuji
PY - 2008
Y1 - 2008
N2 - Initial interaction between rhizobia and legumes actually starts via encounters of both partners in the rhizosphere. In this study, the global expression profiles of Bradyrhizobium japonicum USDA 110 in response to soybean (Glycine max) seed extracts (SSE) and genistein, a major soybean-released isoflavone for nod genes induction of B. japonicum, were compared. SSE induced many genomic loci as compared with genistein (5.0 μM), nevertheless SSE-supplemented medium contained 4.7 μM genistein. SSE markedly induced four predominant genomic regions within a large symbiosis island (681 kb), which include tts genes (type III secretion system) and various nod genes. In addition, SSE-treated cells expressed many genomic loci containing genes for polygalacturonase (cell-wall degradation), exopolysaccharide synthesis, 1-aminocyclopropane-1-carboxyIate deaminase, ribosome proteins family and energy metabolism even outside symbiosis island. On the other hand, genistein-treated cells exclusively showed one expression cluster including common nod gene operon within symbiosis island and six expression loci including multidrug resistance, which were shared with SSE-treated cells. Twelve putatively regulated genes were indeed validated by quantitative RT-PCR. Several SSE-induced genomic loci likely participate in the initial interaction with legumes. Thus, these results can provide a basic knowledge for screening novel genes relevant to the B. japonicum- soybean symbiosis.
AB - Initial interaction between rhizobia and legumes actually starts via encounters of both partners in the rhizosphere. In this study, the global expression profiles of Bradyrhizobium japonicum USDA 110 in response to soybean (Glycine max) seed extracts (SSE) and genistein, a major soybean-released isoflavone for nod genes induction of B. japonicum, were compared. SSE induced many genomic loci as compared with genistein (5.0 μM), nevertheless SSE-supplemented medium contained 4.7 μM genistein. SSE markedly induced four predominant genomic regions within a large symbiosis island (681 kb), which include tts genes (type III secretion system) and various nod genes. In addition, SSE-treated cells expressed many genomic loci containing genes for polygalacturonase (cell-wall degradation), exopolysaccharide synthesis, 1-aminocyclopropane-1-carboxyIate deaminase, ribosome proteins family and energy metabolism even outside symbiosis island. On the other hand, genistein-treated cells exclusively showed one expression cluster including common nod gene operon within symbiosis island and six expression loci including multidrug resistance, which were shared with SSE-treated cells. Twelve putatively regulated genes were indeed validated by quantitative RT-PCR. Several SSE-induced genomic loci likely participate in the initial interaction with legumes. Thus, these results can provide a basic knowledge for screening novel genes relevant to the B. japonicum- soybean symbiosis.
KW - Bradyrhizobium japonicum
KW - Expression clusters
KW - Genistein
KW - Soybean seed extracts
KW - Symbiosis
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U2 - 10.1093/dnares/dsn012
DO - 10.1093/dnares/dsn012
M3 - Article
C2 - 18511436
AN - SCOPUS:56149096008
VL - 15
SP - 201
EP - 214
JO - DNA Research
JF - DNA Research
SN - 1340-2838
IS - 4
ER -