Soybean seed extracts preferentially express genomic loci of bradyrhizobium japonicum in the initial interaction with soybean, glycine max (L.) Merr

W. E.I. Min, Tadashi Yokoyama, Kiwamu Minamisawa, Hisayuki Mitsui, Manabu Itakura, Takakazu Kaneko, Satoshi Tabata, Kazuhiko Saeki, Hirofumi Omori, Shigeyuki Tajima, Toshiki Uchiumi, A. B.E. Mikiko, Takuji Ohwada

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Initial interaction between rhizobia and legumes actually starts via encounters of both partners in the rhizosphere. In this study, the global expression profiles of Bradyrhizobium japonicum USDA 110 in response to soybean (Glycine max) seed extracts (SSE) and genistein, a major soybean-released isoflavone for nod genes induction of B. japonicum, were compared. SSE induced many genomic loci as compared with genistein (5.0 μM), nevertheless SSE-supplemented medium contained 4.7 μM genistein. SSE markedly induced four predominant genomic regions within a large symbiosis island (681 kb), which include tts genes (type III secretion system) and various nod genes. In addition, SSE-treated cells expressed many genomic loci containing genes for polygalacturonase (cell-wall degradation), exopolysaccharide synthesis, 1-aminocyclopropane-1-carboxyIate deaminase, ribosome proteins family and energy metabolism even outside symbiosis island. On the other hand, genistein-treated cells exclusively showed one expression cluster including common nod gene operon within symbiosis island and six expression loci including multidrug resistance, which were shared with SSE-treated cells. Twelve putatively regulated genes were indeed validated by quantitative RT-PCR. Several SSE-induced genomic loci likely participate in the initial interaction with legumes. Thus, these results can provide a basic knowledge for screening novel genes relevant to the B. japonicum- soybean symbiosis.

Original languageEnglish
Pages (from-to)201-214
Number of pages14
JournalDNA Research
Volume15
Issue number4
DOIs
Publication statusPublished - 2008

Keywords

  • Bradyrhizobium japonicum
  • Expression clusters
  • Genistein
  • Soybean seed extracts
  • Symbiosis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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