TY - GEN
T1 - Sorting cells from fluorescent markers organization in confocal microscopy
T2 - 10th International Conference on Image Processing Theory, Tools and Applications, IPTA 2020
AU - Ahmad, Ali
AU - Frindel, Carole
AU - Rousseau, David
N1 - Funding Information:
This work has been supported by project EU H2020 FET Open, PROCHIP, "Chromatin organization PROfiling with high-throughput super-resolution microscopy on a CHIP", grant agreement no. 801336 (https://pro-chip.eu/)
Publisher Copyright:
© 2020 IEEE.
PY - 2020/11/9
Y1 - 2020/11/9
N2 - We consider the problem of sorting cells from fluorescent markers spatial organization via single cell microscopy. This problem is important in bioimaging since this organization can reflect the healthy or pathological state of cells, the chromatin chain configurations or the spatial organization of DNA during main cell cycle. In this work, we address the question of the detection of spatial organization differences according to fluorescent markers density or distribution using textural features. We compare the performances by simulating 3D images and 2D images of the focal plane with various size of the point spread function of the confocal microscope, that is determined by varying the numerical aperture N A of the used objective lens.
AB - We consider the problem of sorting cells from fluorescent markers spatial organization via single cell microscopy. This problem is important in bioimaging since this organization can reflect the healthy or pathological state of cells, the chromatin chain configurations or the spatial organization of DNA during main cell cycle. In this work, we address the question of the detection of spatial organization differences according to fluorescent markers density or distribution using textural features. We compare the performances by simulating 3D images and 2D images of the focal plane with various size of the point spread function of the confocal microscope, that is determined by varying the numerical aperture N A of the used objective lens.
KW - Confocal microscopy
KW - classification
KW - fluorescent markers organization
KW - point spread function
KW - texture
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U2 - 10.1109/IPTA50016.2020.9286463
DO - 10.1109/IPTA50016.2020.9286463
M3 - Conference contribution
AN - SCOPUS:85099290052
T3 - 2020 10th International Conference on Image Processing Theory, Tools and Applications, IPTA 2020
BT - 2020 10th International Conference on Image Processing Theory, Tools and Applications, IPTA 2020
PB - Institute of Electrical and Electronics Engineers Inc.
Y2 - 9 November 2020 through 12 November 2020
ER -