Sorting cells from fluorescent markers organization in confocal microscopy: 3D versus 2D images

Ali Ahmad, Carole Frindel, David Rousseau

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We consider the problem of sorting cells from fluorescent markers spatial organization via single cell microscopy. This problem is important in bioimaging since this organization can reflect the healthy or pathological state of cells, the chromatin chain configurations or the spatial organization of DNA during main cell cycle. In this work, we address the question of the detection of spatial organization differences according to fluorescent markers density or distribution using textural features. We compare the performances by simulating 3D images and 2D images of the focal plane with various size of the point spread function of the confocal microscope, that is determined by varying the numerical aperture N A of the used objective lens.

Original languageEnglish
Title of host publication2020 10th International Conference on Image Processing Theory, Tools and Applications, IPTA 2020
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781728187501
DOIs
Publication statusPublished - 2020 Nov 9
Externally publishedYes
Event10th International Conference on Image Processing Theory, Tools and Applications, IPTA 2020 - Virtual, Paris, France
Duration: 2020 Nov 92020 Nov 12

Publication series

Name2020 10th International Conference on Image Processing Theory, Tools and Applications, IPTA 2020

Conference

Conference10th International Conference on Image Processing Theory, Tools and Applications, IPTA 2020
CountryFrance
CityVirtual, Paris
Period20/11/920/11/12

Keywords

  • Confocal microscopy
  • classification
  • fluorescent markers organization
  • point spread function
  • texture

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Computer Science Applications

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