Sonodynamically induced cell damage and membrane lipid peroxidation by novel porphyrin derivative, DCPH-P-Na(I)

Nagahiko Yumita, Yumiko Iwase, Koji Nishi, Toshihiko Ikeda, Shin Ichiro Umemura, Isao Sakata, Yasunori Momose

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Background: Ultrasonically induced cell damage and active oxygen generation with a novel porphyrin derivative DCPH-P-Na(I), were compared in the same in vitro insonation setup. Materials and Methods: Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound at 2 MHz for up to 60 s in the presence and absence of DCPH-P-Na(I). Cell viability was determined with the trypan blue exclusion test. Lipid peroxidation in cell membranes was estimated by measuring the amount of reactive substance produced immediately following the addition of thiobarbituric acid. Results: Significant enhancement of the rates of both ultrasonically induced cell damage and lipid peroxidation was observed in the presence of 2-8 μM DCPH-P-Na(I). Both rates correlated very well. Conclusion: The enhancement of both rates with DCPH-P-Na(I) was suppressed by 10 mM histidine. These results suggest that ultrasonically generated active oxygen plays a primary role in the ultrasonically induced cell damage in the presence of DCPH-P-Na(I).

Original languageEnglish
Pages (from-to)2241-2246
Number of pages6
JournalAnticancer research
Volume30
Issue number6
Publication statusPublished - 2010 Jun

Keywords

  • Cell damage
  • DCPH-P-Na(I)
  • Lipid peroxidation
  • Reactive oxygen
  • Sonodynamic activation
  • Ultrasound

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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