Sonodynamic therapy with 5-aminolevulinic acid and focused ultrasound for deep-seated intracranial glioma in rat

Tadahiro Ohmura, Takeo Fukushima, Hirotomo Shibaguchi, Shin Yoshizawa, Tooru Inoue, Masahide Kuroki, Kazunari Sasaki, Shin Ichiro Umemura

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46 Citations (Scopus)

Abstract

Background: 5-Aminolevulinic acid (5-ALA) has already been applied clinically as a photosensitizer. In this study, sonodynamically induced selective antitumour effect of 5-ALA for deep-seated lesions was evaluated. Materials and Methods: First, normal rat brains were sonicated via a transducer placed on the dural surface to confirm safe acoustic conditions for normal rat brains. One week after inoculation of brains with C6 rat glioma cells, brains with/without administration of 5-ALA (100 mg/kg body weight) were sonicated. Results: Sonodynamic therapy (SDT) with 5-ALA and focused ultrasound (10 W/cm2, 1.04 MHz, 5 min) achieved selective antitumour effect against deep-seated experimental glioma. Mean tumour sizes in the largest coronal section in sham-operated rats and rats receiving ultrasound with/without 5-ALA were 29.94±10.39, 18.32±5.69 and 30.81±9.65 mm2, respectively. Tumour size was significantly smaller in the SDT group than in other groups (p<0.05). Conclusion: This experimental rat model showed that SDT appears to be useful in the treatment of deep-seated malignant glioma.

Original languageEnglish
Pages (from-to)2527-2534
Number of pages8
JournalAnticancer research
Volume31
Issue number7
Publication statusPublished - 2011 Jul 1

Keywords

  • 5-aminolevulinic acid
  • Deep-seated
  • Focused ultrasound
  • Glioma
  • Rat model
  • Sonodynamic therapy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Ohmura, T., Fukushima, T., Shibaguchi, H., Yoshizawa, S., Inoue, T., Kuroki, M., Sasaki, K., & Umemura, S. I. (2011). Sonodynamic therapy with 5-aminolevulinic acid and focused ultrasound for deep-seated intracranial glioma in rat. Anticancer research, 31(7), 2527-2534.