Sonodynamic effect of erythrosin B on sarcoma 180 cells in vitro

Nagahiko Yumita, Ken ichi Kawabata, Kazuaki Sasaki, Shin ichiro Umemura

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46 Citations (Scopus)


The ultrasonically induced cytotoxic effect of erythrosin B (EB) on isolated sarcoma 180 cells was investigated. The tumor cells were suspended in an air-saturated phosphate buffered saline and exposed to ultrasound at 1.93 MHz in a standing-wave mode for up to 60 s in the presence and absence of EB. The rate of cell damage induction by ultrasound was enhanced by 4-5 times with 160-μM EB, while no cell damage was observed with EB alone. This enhancement was significantly inhibited by histidine. Sonochemical generation of active oxygen species in the presence of EB, measured by ESR spectroscopy, was also inhibited by histidine. These results indicate the involvement of a sonochemical mechanism.

Original languageEnglish
Pages (from-to)259-265
Number of pages7
JournalUltrasonics Sonochemistry
Issue number5
Publication statusPublished - 2002 Oct
Externally publishedYes


  • Cell damage
  • Erythrosin B
  • Sarcoma 180
  • Sonochemical mechanism
  • Ultrasound

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Environmental Chemistry
  • Radiology Nuclear Medicine and imaging
  • Acoustics and Ultrasonics
  • Organic Chemistry
  • Inorganic Chemistry


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