Some adverse actions of chlorothalonil at sublethal levels in rat thymic lymphocytes: Its relation to Zn2+

Mizuki Ikeda, Junji Deguchi, Shota Fukushima, Ai Qingyu, Norihiro Katayama, Hajime Miura, Yasuo Oyama

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Chlorothalonil, a polychlorinated aromatic fungicide, is considered non-toxic to small mammals. However, chlorothalonil inactivates sulfhydryl enzymes and depletes cellular glutathione. Chlorothalonil increases intracellular Zn2+ concentration ([Zn2+]i) in mammalian cells possibly because intracellular Zn2+ is released via zinc-thiol/disulfide interchange. The effects of chlorothalonil at sublethal concentrations on the cellular content of nonprotein thiols ([NPT]i) and [Zn2+]i were examined using flow cytometry in rat thymocytes. Low concentrations (0.3–1 μM) of chlorothalonil increased, but high concentrations (3–10 μM) decreased [NPT]i. These effects of chlorothalonil were partly attenuated by an intracellular Zn2+ chelator. Chlorothalonil at 0.3–10 μM increased [Zn2+]i in a concentration-dependent manner, which was largely dependent on the release of intracellular Zn2+. Both the decrease in [NPT]i and increase in [Zn2+]i increase the vulnerability of cells to oxidative stress. Chlorothalonil at 1–10 μM potentiated the cytotoxicity of H2O2 (300 μM). It was also the case for 10 μM pentachloronitrobenzene, but not 10 μM pentachlorophenol. In conclusion, chlorothalonil at low (sublethal) micromolar concentrations is cytotoxic to mammalian cells under oxidative stress.

Original languageEnglish
Pages (from-to)61-65
Number of pages5
JournalEnvironmental Toxicology and Pharmacology
Volume59
DOIs
Publication statusPublished - 2018 Apr

Keywords

  • Chlorothalonil
  • Cytotoxicity
  • Intracellular Zn
  • Lymphocyte
  • Nonprotein thiol

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

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