TY - JOUR
T1 - Solution structure of agelenin, an insecticidal peptide isolated from the spider Agelena opulenta, and its structural similarities to insect-specific calcium channel inhibitors
AU - Yamaji, Nahoko
AU - Sugase, Kenji
AU - Nakajima, Terumi
AU - Miki, Takafumi
AU - Wakamori, Minoru
AU - Mori, Yasuo
AU - Iwashita, Takashi
PY - 2007/8/7
Y1 - 2007/8/7
N2 - Agelenin, isolated from the Agelenidae spider Agelena opulenta, is a peptide composed of 35 amino acids. We determined the three-dimensional structure of agelenin using two-dimensional NMR spectroscopy. The structure is composed of a short antiparallel β-sheet and four β-turns, which are stabilized by three disulfide bonds. Agelenin has characteristic residues, Phe9, Ser28 and Arg33, which are arranged similarly to the pharmacophore of the insect channel inhibitor, ω-atracotoxin-Hv1a. These observations suggest that agelenin and ω-atracotoxin-Hv1a bind to insect calcium channels in a similar manner. We also suggest that another mode of action may operate in the channel inhibition by ω-agatoxin-IVA and ω-atracotoxin-Hv2a.
AB - Agelenin, isolated from the Agelenidae spider Agelena opulenta, is a peptide composed of 35 amino acids. We determined the three-dimensional structure of agelenin using two-dimensional NMR spectroscopy. The structure is composed of a short antiparallel β-sheet and four β-turns, which are stabilized by three disulfide bonds. Agelenin has characteristic residues, Phe9, Ser28 and Arg33, which are arranged similarly to the pharmacophore of the insect channel inhibitor, ω-atracotoxin-Hv1a. These observations suggest that agelenin and ω-atracotoxin-Hv1a bind to insect calcium channels in a similar manner. We also suggest that another mode of action may operate in the channel inhibition by ω-agatoxin-IVA and ω-atracotoxin-Hv2a.
KW - Agelenin
KW - Insect calcium channel
KW - NMR
KW - Spider toxin
KW - Structure
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U2 - 10.1016/j.febslet.2007.06.077
DO - 10.1016/j.febslet.2007.06.077
M3 - Article
C2 - 17644092
AN - SCOPUS:34547124845
VL - 581
SP - 3789
EP - 3794
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 20
ER -