Solubilization and high activity of enzymes in ionic liquids by modification with comb-shaped poly (ethylene glycol)

Kazunori Nakashima, Tatsuo Maruyama, Noriho Kamiya, Masahiro Goto

Research output: Contribution to conferencePaperpeer-review

Abstract

Subtilisin Carlsberg was modified with comb-shaped poly (ethylene glycol), PM13. PM13 modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs, whereas native subtilisin was not soluble in any ILs. Furthermore, PM13-Sub dissolved in ILs exhibited the excellent catalytic activity in transesterification of N-acetyl-L-phenylalanine ethyl ester with 1-butanol. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.

Original languageEnglish
Pages4647-4648
Number of pages2
Publication statusPublished - 2005
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Keywords

  • Biotransformation
  • Dissolution
  • Enzymatic reaction
  • Ionic liquids
  • Protein
  • Solubilization

ASJC Scopus subject areas

  • Engineering(all)

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