TY - JOUR
T1 - SOG1, a plant-specific master regulator of DNA damage responses, originated from nonvascular land plants
AU - Sakamoto, Ayako N.
AU - Sakamoto, Tomoaki
AU - Yokota, Yuichiro
AU - Teranishi, Mika
AU - Yoshiyama, Kaoru O.
AU - Kimura, Seisuke
N1 - Funding Information:
We are most grateful to Fabien Nogué (INRAE, Versailles) for providing materials and for critically reading the manuscript. We also thank Norihiko Yagi and Naoko Maeda for the technical assistance. This work was partially supported by grants-in-aid from the Japan Society for the Promotion of Science (JSPS) KAKENHI grant numbers 17K00561, 19K12332, and 20K12174 to A.N.S. and Y.Y.; 19K12317 to M.T.; 20K06697 to K.O.Y.; and 21H02513 to S.K. It was also supported by QST Diversity Promotion Grant 2020-4 to A.N.S. and the MEXT-Supported Program for the Strategic Research Foundation at Private Universities (grant number S1511023) from the Ministry of Education, Culture, Sports, Science and Technology of Japan to S.K.
Funding Information:
We are most grateful to Fabien Nogué (INRAE, Versailles) for providing materials and for critically reading the manuscript. We also thank Norihiko Yagi and Naoko Maeda for the technical assistance. This work was partially supported by grants‐in‐aid from the Japan Society for the Promotion of Science (JSPS) KAKENHI grant numbers 17K00561, 19K12332, and 20K12174 to A.N.S. and Y.Y.; 19K12317 to M.T.; 20K06697 to K.O.Y.; and 21H02513 to S.K. It was also supported by QST Diversity Promotion Grant 2020‐4 to A.N.S. and the MEXT‐Supported Program for the Strategic Research Foundation at Private Universities (grant number S1511023) from the Ministry of Education, Culture, Sports, Science and Technology of Japan to S.K.
Publisher Copyright:
© 2021 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.
PY - 2021/12
Y1 - 2021/12
N2 - The suppressor of gamma response 1 (SOG1), a NAM, ATAF1, 2, and CUC2 (NAC)-type transcription factor found in seed plants, is a master regulator of DNA damage responses (DDRs). Upon DNA damage, SOG1 regulates the expression of downstream DDR genes. To know the origin of the DDR network in land plants, we searched for a homolog(s) of SOG1 in a moss Physcomitrium (Physcomitrella) patens and identified PpSOG1a and PpSOG1b. To assess if either or both of them function(s) in DDR, we knocked out the PpSOG1s using CRISPR/Cas9-mediated gene editing and analyzed the responses to DNA-damaging treatments. The double-knockout (KO) sog1a sog1b plants showed resistance to γ-rays, bleomycin, and ultraviolet B (UVB) treatments similarly seen in Arabidopsis sog1 plants. Next, we irradiated wild-type (WT) and KO plants with γ-rays and analyzed the whole transcriptome to examine the effect on the expression of DDR genes. The results revealed that many P. patens genes involved in the checkpoint, DNA repair, replication, and cell cycle-related genes were upregulated after γ-irradiation, which was not seen in sog1a sog1b plant. These results suggest that PpSOG1a and PpSOG1b work redundantly on DDR response in P. patens; in addition, plant-specific DDR systems had been established before the emergence of vascular plants.
AB - The suppressor of gamma response 1 (SOG1), a NAM, ATAF1, 2, and CUC2 (NAC)-type transcription factor found in seed plants, is a master regulator of DNA damage responses (DDRs). Upon DNA damage, SOG1 regulates the expression of downstream DDR genes. To know the origin of the DDR network in land plants, we searched for a homolog(s) of SOG1 in a moss Physcomitrium (Physcomitrella) patens and identified PpSOG1a and PpSOG1b. To assess if either or both of them function(s) in DDR, we knocked out the PpSOG1s using CRISPR/Cas9-mediated gene editing and analyzed the responses to DNA-damaging treatments. The double-knockout (KO) sog1a sog1b plants showed resistance to γ-rays, bleomycin, and ultraviolet B (UVB) treatments similarly seen in Arabidopsis sog1 plants. Next, we irradiated wild-type (WT) and KO plants with γ-rays and analyzed the whole transcriptome to examine the effect on the expression of DDR genes. The results revealed that many P. patens genes involved in the checkpoint, DNA repair, replication, and cell cycle-related genes were upregulated after γ-irradiation, which was not seen in sog1a sog1b plant. These results suggest that PpSOG1a and PpSOG1b work redundantly on DDR response in P. patens; in addition, plant-specific DDR systems had been established before the emergence of vascular plants.
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U2 - 10.1002/pld3.370
DO - 10.1002/pld3.370
M3 - Article
AN - SCOPUS:85121869128
VL - 5
JO - Plant Direct
JF - Plant Direct
SN - 2475-4455
IS - 12
M1 - e370
ER -