TY - JOUR
T1 - Sodium carboxymethyl cellulose-based extrafibrillar demineralization to optimize dentin bonding durability
AU - Zhao, Yaning
AU - Yu, Jian
AU - Guo, Jingmei
AU - Yang, Hongye
AU - Huang, Cui
N1 - Funding Information:
This work was financially supported by National Natural Science Foundation of China ( 81701012 and 81901043 ) and Postdoctoral Science Foundation of China ( 2018M640735 ).
Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2022/12
Y1 - 2022/12
N2 - Objective: To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms. Methods: The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration. A single layer collagen mineralization/demineralization model was designed to visualize extrafibrillar demineralization in detail. Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA). The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized. To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique. The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging. The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated. Results: After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure. The mechanical properties of SCMC-conditioned dentin were largely preserved. Compared with PA, SCMC conditioning produced greater MTBS values and less nanoleakage expression after aging. Endogenous gelatinolytic activity was suppressed in SCMC-conditioned dentin. In addition to being nontoxic, the inhibition of rhMMP-2 by SCMC was confirmed to be dose-dependent. Significance: From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.
AB - Objective: To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms. Methods: The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration. A single layer collagen mineralization/demineralization model was designed to visualize extrafibrillar demineralization in detail. Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA). The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized. To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique. The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging. The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated. Results: After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure. The mechanical properties of SCMC-conditioned dentin were largely preserved. Compared with PA, SCMC conditioning produced greater MTBS values and less nanoleakage expression after aging. Endogenous gelatinolytic activity was suppressed in SCMC-conditioned dentin. In addition to being nontoxic, the inhibition of rhMMP-2 by SCMC was confirmed to be dose-dependent. Significance: From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.
KW - Dentin bonding
KW - Extrafibrillar demineralization
KW - Matrix metalloproteinases
KW - Sodium carboxymethyl cellulose
UR - http://www.scopus.com/inward/record.url?scp=85142803525&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85142803525&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2022.11.001
DO - 10.1016/j.dental.2022.11.001
M3 - Article
C2 - 36446650
AN - SCOPUS:85142803525
SN - 0109-5641
VL - 38
SP - 2096
EP - 2114
JO - Dental Materials
JF - Dental Materials
IS - 12
ER -