Sodium and chloride ion-dependent transport of β-alanine across the blood-brain barrier

Junko Komura, Ikumi Tamai, Mizuho Senmaru, Tetsuya Terasaki, Yoshimichi Sai, Akira Tsuji

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


The characteristics of β-alanine transport at the blood-brain barrier were studied by using primary cultured bovine brain capillary endothelial cells. Kinetic analysis of the β-[3H]alanine transport indicated that the transporter for β-alanine functions with K(t) of 25.3 ± 2.5 μM and J(max) of 6.90 ± 0.48 nmol/30 min/mg of protein in the brain capillary endothelial cells. β-[3H]Alanine uptake is mediated by an active transporter, because metabolic inhibitors (2,4-dinitrophenol and NaN3) and low temperature reduced the uptake significantly. Furthermore, the uptake of β-[3H]alanine required Na+ and Cl- in the external medium. Stoichiometric analysis of the transport demonstrated that two sodium ions and one chloride ion are associated with one β-alanine molecule. The Na+ and Cl- dependent uptake of β-[3H]alanine was stimulated by a valinomycin-induced inside-negative K+-diffusion potential. β-Amino acids (β-alanine, taurine, and hypotaurine) inhibited strongly the uptake of β-[3H]alanine, whereas α- and γ-amino acids had little or no inhibitory effect. In ATP-depleted cells, the uptake of β[3H]alanine was stimulated by preloading of β-alanine or taurine but not L-leucine. These results show that β-alanine is taken up by brain capillary endothelial cells, via the secondary active transport mechanism that is common to β-amino acids.

Original languageEnglish
Pages (from-to)330-335
Number of pages6
JournalJournal of Neurochemistry
Issue number1
Publication statusPublished - 1996 Jul


  • Brain active transporter
  • Brain capillary endothelial cell
  • Carrier- mediated uptake
  • Taurine
  • β-Alanine
  • β-Amino acid

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Sodium and chloride ion-dependent transport of β-alanine across the blood-brain barrier'. Together they form a unique fingerprint.

Cite this