SNAP-23 is not essential for constitutive exocytosis in HeLa cells

Miki Okayama, Toshiya Arakawa, Itaru Mizoguchi, Yoshifumi Tajima, Taishin Takuma

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

We applied the small interfering RNA (siRNA) technique and over-expression of a dominant-negative mutant to evaluate the role of SNAP-23, a non-neuronal isoform of SNAP-25, in constitutive exocytosis from HeLa cells. Although the protein level of SNAP-23 was reduced to less than 10% of the control value by siRNA directed against SNAP-23, exocytosis of SEAP (secreted alkaline phosphatase) was normal. Double knockdown of SNAP-23 and syntaxin-4 also failed to inhibit the secretion. Furthermore, over-expression of δC8-SNAP-23, a dominant-negative mutant of SNAP-23, did not abrogate SEAP secretion. These results suggest that SNAP-23 is not essential for constitutive exocytosis of SEAP.

Original languageEnglish
Pages (from-to)4583-4588
Number of pages6
JournalFEBS Letters
Volume581
Issue number24
DOIs
Publication statusPublished - 2007 Oct 2

Keywords

  • Constitutive exocytosis
  • Dominant-negative mutant
  • SEAP
  • SNAP-23
  • siRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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