TY - JOUR
T1 - SNAP-23 is not essential for constitutive exocytosis in HeLa cells
AU - Okayama, Miki
AU - Arakawa, Toshiya
AU - Mizoguchi, Itaru
AU - Tajima, Yoshifumi
AU - Takuma, Taishin
N1 - Funding Information:
We wish to thank Prof. Yosuke Tojyo and Dr. Akihiko Tanimura, Department of Pharmacology, Health Sciences University, for helpful discussion. This work was supported in part by the Academic Sciences Frontier Project and by a grant-in-aid for scientific research (No. 17591945 to T.T.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2007/10/2
Y1 - 2007/10/2
N2 - We applied the small interfering RNA (siRNA) technique and over-expression of a dominant-negative mutant to evaluate the role of SNAP-23, a non-neuronal isoform of SNAP-25, in constitutive exocytosis from HeLa cells. Although the protein level of SNAP-23 was reduced to less than 10% of the control value by siRNA directed against SNAP-23, exocytosis of SEAP (secreted alkaline phosphatase) was normal. Double knockdown of SNAP-23 and syntaxin-4 also failed to inhibit the secretion. Furthermore, over-expression of δC8-SNAP-23, a dominant-negative mutant of SNAP-23, did not abrogate SEAP secretion. These results suggest that SNAP-23 is not essential for constitutive exocytosis of SEAP.
AB - We applied the small interfering RNA (siRNA) technique and over-expression of a dominant-negative mutant to evaluate the role of SNAP-23, a non-neuronal isoform of SNAP-25, in constitutive exocytosis from HeLa cells. Although the protein level of SNAP-23 was reduced to less than 10% of the control value by siRNA directed against SNAP-23, exocytosis of SEAP (secreted alkaline phosphatase) was normal. Double knockdown of SNAP-23 and syntaxin-4 also failed to inhibit the secretion. Furthermore, over-expression of δC8-SNAP-23, a dominant-negative mutant of SNAP-23, did not abrogate SEAP secretion. These results suggest that SNAP-23 is not essential for constitutive exocytosis of SEAP.
KW - Constitutive exocytosis
KW - Dominant-negative mutant
KW - SEAP
KW - SNAP-23
KW - siRNA
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U2 - 10.1016/j.febslet.2007.08.046
DO - 10.1016/j.febslet.2007.08.046
M3 - Article
C2 - 17825825
AN - SCOPUS:34548665740
SN - 0014-5793
VL - 581
SP - 4583
EP - 4588
JO - FEBS Letters
JF - FEBS Letters
IS - 24
ER -