SMAP2 Regulates Retrograde Transport from Recycling Endosomes to the Golgi

Tatsuyuki Matsudaira, Yasunori Uchida, Kenji Tanabe, Shunsuke Kon, Toshio Watanabe, Tomohiko Taguchi, Hiroyuki Arai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Retrograde transport is where proteins and lipids are transported back from the plasma membrane (PM) and endosomes to the Golgi, and crucial for a diverse range of cellular functions. Recycling endosomes (REs) serve as a sorting station for the retrograde transport and we recently identified evection-2, an RE protein with a pleckstrin homology (PH) domain, as an essential factor of this pathway. How evection-2 regulates retrograde transport from REs to the Golgi is not well understood. Here, we report that evection-2 binds to SMAP2, an Arf GTPase-activating protein. Endogenous SMAP2 localized mostly in REs and to a lesser extent, the trans-Golgi network (TGN). SMAP2 binds evection-2, and the RE localization of SMAP2 was abolished in cells depleted of evection-2. Knockdown of SMAP2, like that of evection-2, impaired the retrograde transport of cholera toxin B subunit (CTxB) from REs. These findings suggest that evection-2 recruits SMAP2 to REs, thereby regulating the retrograde transport of CTxB from REs to the Golgi.

Original languageEnglish
Article numbere69145
JournalPloS one
Volume8
Issue number7
DOIs
Publication statusPublished - 2013 Jul 8

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Matsudaira, T., Uchida, Y., Tanabe, K., Kon, S., Watanabe, T., Taguchi, T., & Arai, H. (2013). SMAP2 Regulates Retrograde Transport from Recycling Endosomes to the Golgi. PloS one, 8(7), [e69145]. https://doi.org/10.1371/journal.pone.0069145