Small heat shock protein Hsp27 directly binds to alpha/beta tubulin heterodimer and inhibits DMSO-induced tubulin polymerization

Mizuki Hino, Hiroshi Hosoya

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, the interaction between the small heat shock protein Hsp27 and α/β tubulin heterodimer was investigated. First, immunoprecipitation analysis using recombinant Hsp27 and purified tubulin indicated that Hsp27 binds with α/β tubulin heterodimer. Next, the effect of Hsp27 on tubulin polymerization was examined. Hsp27 showed the inhibitory effect on dimethylsulfoxide (DMSO)-induced tubulin polymerization. The inhibitory effect was dose-dependent: about 30% inhibition of polymerization was found after addition of 0.85 mg/mL of Hsp27, 70% inhibition was found by 3.4 mg/mL of Hsp27.

Original languageEnglish
Pages (from-to)27-30
Number of pages4
JournalBiomedical Research
Volume24
Issue number1
DOIs
Publication statusPublished - 2003
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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