Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells

Mizuki Hino, Kazuki Kurogi, Masa Aki Okubo, Maki Murata-Hori, Hiroshi Hosoya

Research output: Contribution to journalArticlepeer-review

84 Citations (Scopus)

Abstract

One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both α- and β-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalBiochemical and biophysical research communications
Volume271
Issue number1
DOIs
Publication statusPublished - 2000 Apr 29
Externally publishedYes

Keywords

  • HSP27
  • Microtubules
  • Mitotic spindle
  • Monoclonal antibody
  • Small heat shock protein
  • Tubulin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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