Small GTPase Rho regulates thrombin-induced platelet aggregation

Hiroaki Nishioka, Hisanori Horiuchi, Arata Tabuchi, Akira Yoshioka, Ryutaro Shirakawa, Toru Kita

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Platelets play essential roles in hemostasis and thrombosis by aggregating with each other. However, the molecular mechanism governing platelet aggregation is not yet fully understood. Here, we established an assay system using platelets permeabilized with streptolysin-O to analyze mechanism of the thrombin-induced aggregation, focusing upon a controversial issue in the field whether small GTPase Rho regulates the aggregation. Incubation of the permeabilized platelets with Rho GDP-dissociation inhibitor, an inhibitory regulator for Rho family GTPases, extracted Rho family proteins extensively from the plasma and intracellular membranes, and inhibited the thrombin-induced aggregation. Incubation of the permeabilized platelets with botulinum exoenzyme C3, which specifically inhibits Rho function by ADP-ribosylating it, abolished the thrombin-induced aggregation. Thus, Rho is involved in thrombin-induced aggregation of platelets.

Original languageEnglish
Pages (from-to)970-975
Number of pages6
JournalBiochemical and biophysical research communications
Volume280
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Aggregation
  • C3
  • Platelet
  • Rho
  • RhoGDI
  • Thrombin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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