Small GTPase Rab39A interacts with UACA and regulates the retinoic acid-induced neurite morphology of Neuro2A cells

Yasunori Mori, Takahide Matsui, Daisuke Omote, Mitsunori Fukuda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We screened for a Rab39-specific effector by performing a yeast two-hybrid assay with GTP-locked Rab39A/B as the bait and identified UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) as a specific Rab39A/B-binding protein. Deletion analysis revealed that a C-terminal coiled-coil domain of UACA functions as a GTP-dependent Rab39-binding domain. shRNA-mediated knockdown of endogenous Rab39A or UACA in mouse neuroblastoma Neuro2A cells resulted in a change in retinoic acid-induced neurite morphology from a multipolar morphology to a bipolar morphology. Taken together, these findings indicate that UACA functions as a Rab39A effector in the retinoic acid-induced differentiation of Neuro2A cells.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalBiochemical and biophysical research communications
Volume435
Issue number1
DOIs
Publication statusPublished - 2013 May 24

Keywords

  • Membrane traffic
  • Neuritogenesis
  • Neuro2A cells
  • Rab39 effector
  • Small GTPase
  • UACA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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