SLA-DRB1 and -DQB1 genotyping by the PCR-SSOP-Luminex method

A. Ando, A. Shigenari, M. Ota, M. Sada, H. Kawata, F. Azuma, C. Kojima-Shibata, M. Nakajoh, K. Suzuki, H. Uenishi, J. K. Kulski, Hidetoshi Inoko

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    A simple and novel genotyping method was developed to detect alleles at the swine leukocyte antigen (SLA)-DRB1 and -DQB1 class II loci by using polymerase chain reaction (PCR)-fluorescently labeled sequence-specific oligonucleotide probes (SSOPs) and Luminex 100 xMAP detection. The PCR-SSOP-Luminex method exhibited accuracy of 95% for both SLA-DRB1 and -DQB1 in 6 homozygous and 16 heterozygous pig samples as confirmed by sequencing the PCR products of the same samples. In addition, 12 low-resolution SLA class II haplotypes consisting of 7 and 9 DRB1 and DQB1 alleles were identified, respectively, in one population of 283 Landrace pigs. This genotyping method facilitates the rapid and accurate identification of two- or four-digit alleles at the SLA-DRB1 and -DQB1 loci.

    Original languageEnglish
    Pages (from-to)49-55
    Number of pages7
    JournalTissue Antigens
    Volume78
    Issue number1
    DOIs
    Publication statusPublished - 2011 Jul

    Keywords

    • Genotyping
    • Luminex method
    • Major histocompatibility complex
    • Polymerase chain reaction-sequence-specific oligonucleotide probe
    • Swine
    • Swine leukocyte antigen

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology
    • Biochemistry
    • Genetics

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