Skeletal muscle tissue improvement by co-culture system in gelatin methacrylate hydrogel

Serge Jacques Pierre Ostrovidow, Samad Ahadian, Hirokazu Kaji, M. Ramalingam, A. Khademhosseini

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we demonstrate that a co-culture approach between C2C12 myoblasts and pheochromocytoma PC12 neuronal cells allows for better formation of muscle-like tissues than a mono-culture of C2C12 cells. In particular, we used a micromolding technique to fabricate microgrooved patterns of methacrylated gelatin (GelMA) hydrogel, which served as a substrate to culture encapsulated cells in GelMA and to induce myoblasts alignment. GelMA is a semi-natural polymer that is easy to pattern by UV irradiation for making 3D structures in which cells can proliferate. Myoblasts and the resulting myotubes were analyzed in terms of alignment and length, respectively, in the mono-culture and the co-culture systems for both patterned and unpatterned regions.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1135-1137
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • C2C12
  • Co-culture
  • Gelma
  • Hydrogel
  • PC12

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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