TY - GEN
T1 - Skeletal muscle tissue improvement by co-culture system in gelatin methacrylate hydrogel
AU - Ostrovidow, Serge Jacques Pierre
AU - Ahadian, Samad
AU - Kaji, Hirokazu
AU - Ramalingam, M.
AU - Khademhosseini, A.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - In this study, we demonstrate that a co-culture approach between C2C12 myoblasts and pheochromocytoma PC12 neuronal cells allows for better formation of muscle-like tissues than a mono-culture of C2C12 cells. In particular, we used a micromolding technique to fabricate microgrooved patterns of methacrylated gelatin (GelMA) hydrogel, which served as a substrate to culture encapsulated cells in GelMA and to induce myoblasts alignment. GelMA is a semi-natural polymer that is easy to pattern by UV irradiation for making 3D structures in which cells can proliferate. Myoblasts and the resulting myotubes were analyzed in terms of alignment and length, respectively, in the mono-culture and the co-culture systems for both patterned and unpatterned regions.
AB - In this study, we demonstrate that a co-culture approach between C2C12 myoblasts and pheochromocytoma PC12 neuronal cells allows for better formation of muscle-like tissues than a mono-culture of C2C12 cells. In particular, we used a micromolding technique to fabricate microgrooved patterns of methacrylated gelatin (GelMA) hydrogel, which served as a substrate to culture encapsulated cells in GelMA and to induce myoblasts alignment. GelMA is a semi-natural polymer that is easy to pattern by UV irradiation for making 3D structures in which cells can proliferate. Myoblasts and the resulting myotubes were analyzed in terms of alignment and length, respectively, in the mono-culture and the co-culture systems for both patterned and unpatterned regions.
KW - C2C12
KW - Co-culture
KW - Gelma
KW - Hydrogel
KW - PC12
UR - http://www.scopus.com/inward/record.url?scp=84901793198&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901793198&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901793198
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1135
EP - 1137
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -