Size-based chromatography of signaling clusters in a living cell membrane

Niña G. Caculitan, Hiroyuki Kai, Eulanca Y. Liu, Nicole Fay, Yan Yu, Theobald Lohmüller, Geoff P. O'Donoghue, Jay T. Groves

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Here we introduce a form of chromatography that can be imposed on the membrane of a living cell. A cell-cell signaling interaction is reconstituted in a hybrid live cell-supported membrane junction. The chromatographic material consists of a hexagonally ordered array of gold nanoparticles (nanodot array), which is fabricated onto the underlying substrate. While individual membrane components move freely throughout the array, the movement of larger assemblies is impeded if they exceed the physical dimensions of the array. This tactile approach to probing membrane structures in living cells reveals organizational aspects of the membrane environment unobservable by other techniques.

Original languageEnglish
Pages (from-to)2293-2298
Number of pages6
JournalNano Letters
Issue number5
Publication statusPublished - 2014 May 14
Externally publishedYes


  • Membrane
  • T cell receptor
  • cluster chromatography
  • nanodot array

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering


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