Site-specific covalent modification of RNA guided by functionality-transfer oligodeoxynucleotides

Kazumitsu Onizuka, Yosuke Taniguchi, Shigeki Sasaki

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Efficient methods for the covalent modification of large RNA molecules should find significance utility as innovative biological tools as well as therapeutic methods. In this study, the development of a general method for site-specific RNA modification guided by the functional ODN template has been investigated. The ODN probe containing 6-thioguanosine was modified by the methylenediketone derivative to form the S-functionalized ODN. Site-specific and cytosine-selective RNA modifications were achieved by the functionality- transfer reaction from the sulfur atom of the functionalized probe to the amino group of the cytosine base of the target strand. It was shown that the base and site selectivity were due to the close proximity of the reactants in the DNA-RNA duplexes.

Original languageEnglish
Pages (from-to)799-803
Number of pages5
JournalBioconjugate chemistry
Volume20
Issue number4
DOIs
Publication statusPublished - 2009 Apr 15
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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