Single‐channel recordings of chloride currents in primary cultured Drosophila neurons

Daisuke Yamamoto, Nobuyuki Suzuki

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Single‐chloride‐channel currents were recorded from primary cultured Drosophila neurons by means of the gigaohm‐seal patch‐clamp technique. Small inward‐going current channels were observed in excised inside‐out patches with the external face of the membrane exposed to bathing solutions devoid of K+, Na+, and Ca2+. The inward current was affected by changing the anions but not the cations bathing the cytoplasmic face of the patch. Complete replacement of CI by glutamate eliminated the current. The current was maintained with intracellular solutions containing NO3 in place of CI. The single‐channel conductance was estimated to be 7 ps with CI, and 11 ps with NO3 at 10°C. Possible functions of this anion‐selective channel have been discussed.

    Original languageEnglish
    Pages (from-to)151-158
    Number of pages8
    JournalArchives of Insect Biochemistry and Physiology
    Volume6
    Issue number3
    DOIs
    Publication statusPublished - 1987 Nov

    Keywords

    • CI
    • Drosophila
    • anion channel
    • neurons
    • patch‐clamp

    ASJC Scopus subject areas

    • Physiology
    • Biochemistry
    • Insect Science

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