Single-seed PCR-RFLP analysis for the identification of S haplotypes in commercial F1 hybrid cultivars of broccoli and cabbage

K. Sakamoto, M. Kusaba, T. Nishio

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    39 Citations (Scopus)

    Abstract

    Several simple methods of DNA preparation from plant tissues were evaluated for PCR-RFLP analyses of SLG and SRK alleles, which can be used for the identification of S haplotypes of breeding lines in broccoli and cabbage (Brassica oleracea L.) and in purity tests of F1 hybrid seeds. On the five methods tested, the NaI method was found to be the most suitable for the amplification of the SLG and SRK alleles. This method enables the use of a single seed as testing material. Using this method, we identified S haplotypes of 31 broccoli and 31 cabbage cultivars. Ninety-four percent of the cultivars of broccoli and 97% of those of cabbage were-single cross F1 hybrids. Nine and 15 S haplotypes were found in broccoli and cabbage, respectively. The small number of S haplotypes in broccoli suggests the importance of incorporating new S haplotypes in the breeding program.

    Original languageEnglish
    Pages (from-to)400-406
    Number of pages7
    JournalPlant Cell Reports
    Volume19
    Issue number4
    DOIs
    Publication statusPublished - 2000 Mar

    Keywords

    • Brassica
    • DNA polymorphism
    • S haplotype
    • Seed purity test
    • Self-incompatibility

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Plant Science

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