Single-protein study of photoresistance of pigment-protein complex in lipid bilayer

Daisuke Uchiyama, Hajime Hoshino, Kohei Otomo, Taro Kato, Ken Ichi Onda, Akira Watanabe, Hiroyuki Oikawa, Satoru Fujiyoshi, Michio Matsushita, Mamoru Nango, Natsuko Watanabe, Ayumi Sumino, Takehisa Dewa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Photoresistance of a pigment-binding membrane protein, light-harvesting 2 (LH2) complex from the photosynthetic bacterium, Rhodopseudomonas acidophila, was investigated by fluorescence of single LH2 complexes at a temperature of 296 K. Before irreversibly stopping fluorescence, a single LH2 complex in phospholipid bilayer of dimyristoylphosphatidylcholine (DMPC) emitted on average 4 times more fluorescence photons than a complex in detergent micelle of octylglucopyranoside (OG). Fluorescence-excitation spectrum of single LH2 complexes taken at 5 K showed that the LH2 complex is structurally less perturbed in DMPC bilayer than in OG micelle, suggesting that structural instability reduces photoresistance of LH2.

Original languageEnglish
Pages (from-to)135-137
Number of pages3
JournalChemical Physics Letters
Volume511
Issue number1-3
DOIs
Publication statusPublished - 2011 Jul 26

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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