Single-Molecule Visualization of the Activity of a Zn2+-Dependent DNAzyme

Masayuki Endo, Yosuke Takeuchi, Yuki Suzuki, Tomoko Emura, Kumi Hidaka, Fuan Wang, Itamar Willner, Hiroshi Sugiyama

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

We demonstrate the single-molecule imaging of the catalytic reaction of a Zn2+-dependent DNAzyme in a DNA origami nanostructure. The single-molecule catalytic activity of the DNAzyme was examined in the designed nanostructure, a DNA frame. The DNAzyme and a substrate strand attached to two supported dsDNA molecules were assembled in the DNA frame in two different configurations. The reaction was monitored by observing the configurational changes of the incorporated DNA strands in the DNA frame. This configurational changes were clearly observed in accordance with the progress of the reaction. The separation processes of the dsDNA molecules, as induced by the cleavage by the DNAzyme, were directly visualized by high-speed atomic force microscopy (AFM). This nanostructure-based AFM imaging technique is suitable for the monitoring of various chemical and biochemical catalytic reactions at the single-molecule level.

Original languageEnglish
Pages (from-to)10550-10554
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number36
DOIs
Publication statusPublished - 2015 Sep 1
Externally publishedYes

Keywords

  • DNA origami
  • DNA structures
  • DNAzymes
  • high-speed atomic force microscopy
  • single-molecule imaging

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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