Single-molecule speckle analysis of actin filament turnover in lamellipodia

Naoki Watanabe, Timothy J. Mitchison

    Research output: Contribution to journalArticlepeer-review

    335 Citations (Scopus)

    Abstract

    Lamellipodia are thin, veil-like extensions at the edge of cells that contain a dynamic array of actin filaments. We describe an approach for analyzing spatial regulation of actin polymerization and depolymerization in vivo in which we tracked single molecules of actin fused to the green fluorescent protein. Polymerization and the lifetime of actin filaments in lamellipodia were measured with high spatial precision. Basal polymerization and depolymerization occurred throughout lamellipodia with largely constant kinetics, and polymerization was promoted within one micron of the lamellipodium tip. Most of the actin filaments in the lamellipodium were generated by polymerization away from the tip.

    Original languageEnglish
    Pages (from-to)1083-1086
    Number of pages4
    JournalScience
    Volume295
    Issue number5557
    DOIs
    Publication statusPublished - 2002 Feb 8

    ASJC Scopus subject areas

    • General

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