Single molecule nanomanipulation of biomolecules

Yoshiharu Ishii, Akihiko Ishijima, Toshio Yanagida

Research output: Contribution to journalReview articlepeer-review

59 Citations (Scopus)


The development of nanomanipulation techniques has given investigators the ability to manipulate single biomolecules and to record mechanical events of biomolecules at the single molecule level. The techniques were developed to elucidate the mechanism of molecular motors. We can directly monitor the unitary process of the mechanical work and the energy conversion processes by combining these techniques with the single molecule imaging techniques. Our results strongly suggest that the sliding movement of the actomyosin motor is driven by Brownian movement. Other groups have reported data that are more consistent with the lever arm model. These methods and imaging techniques enable us to monitor the behavior of biomolecules at work and will be applied to other molecular machines.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalTrends in Biotechnology
Issue number6
Publication statusPublished - 2001 Jun 1
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


Dive into the research topics of 'Single molecule nanomanipulation of biomolecules'. Together they form a unique fingerprint.

Cite this