TY - CHAP
T1 - Single-molecule measurements using microneedles
AU - Yanagida, Toshio
AU - Ishii, Yoshiharu
AU - Ishijima, Akihiko
PY - 2011/10/24
Y1 - 2011/10/24
N2 - Myosin is both an enzyme and a molecular motor that hydrolyzes ATP and interacts with actin filaments for force generation. Manipulation techniques with microneedles and laser traps have recently been developed to capture and manipulate the actomyosin interaction for the purpose of revealing the mechanics of this system. Combined with single-molecule imaging techniques, the coupling between chemical processes (ATP hydrolysis) and mechanical processes (myosin force generation) has been directly determined. In this chapter, we describe these two manipulation techniques, especially microneedle method, in detail.
AB - Myosin is both an enzyme and a molecular motor that hydrolyzes ATP and interacts with actin filaments for force generation. Manipulation techniques with microneedles and laser traps have recently been developed to capture and manipulate the actomyosin interaction for the purpose of revealing the mechanics of this system. Combined with single-molecule imaging techniques, the coupling between chemical processes (ATP hydrolysis) and mechanical processes (myosin force generation) has been directly determined. In this chapter, we describe these two manipulation techniques, especially microneedle method, in detail.
KW - Laser trap
KW - Mechanochemical -coupling
KW - Microneedle
KW - Myosin
KW - Single-molecule imaging
KW - Single-molecule manipulation
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U2 - 10.1007/978-1-61779-261-8_10
DO - 10.1007/978-1-61779-261-8_10
M3 - Chapter
C2 - 21809205
AN - SCOPUS:80054720859
SN - 9781617792601
T3 - Methods in Molecular Biology
SP - 143
EP - 159
BT - Single Molecule Enzymology
A2 - Mashanov, Gregory I.
A2 - Batters, Christopher
ER -