Single-molecule measurements using microneedles

Toshio Yanagida; Yoshiharu Ishii; Akihiko Ishijima

doi:10.1007/978-1-61779-261-8_10

Single-molecule measurements using microneedles

Toshio Yanagida, Yoshiharu Ishii, Akihiko Ishijima

Institute of Multidisciplinary Research for Advanced Materials (IMRAM)

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Myosin is both an enzyme and a molecular motor that hydrolyzes ATP and interacts with actin filaments for force generation. Manipulation techniques with microneedles and laser traps have recently been developed to capture and manipulate the actomyosin interaction for the purpose of revealing the mechanics of this system. Combined with single-molecule imaging techniques, the coupling between chemical processes (ATP hydrolysis) and mechanical processes (myosin force generation) has been directly determined. In this chapter, we describe these two manipulation techniques, especially microneedle method, in detail.

Original language	English
Title of host publication	Single Molecule Enzymology
Subtitle of host publication	Methods and Protocols
Editors	Gregory I. Mashanov, Christopher Batters
Pages	143-159
Number of pages	17
DOIs	https://doi.org/10.1007/978-1-61779-261-8_10
Publication status	Published - 2011 Oct 24

Publication series

Name	Methods in Molecular Biology
Volume	778
ISSN (Print)	1064-3745

Keywords

Laser trap
Mechanochemical -coupling
Microneedle
Myosin
Single-molecule imaging
Single-molecule manipulation

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-61779-261-8_10

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