Single-molecule measurements using microneedles

Toshio Yanagida, Yoshiharu Ishii, Akihiko Ishijima

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Myosin is both an enzyme and a molecular motor that hydrolyzes ATP and interacts with actin filaments for force generation. Manipulation techniques with microneedles and laser traps have recently been developed to capture and manipulate the actomyosin interaction for the purpose of revealing the mechanics of this system. Combined with single-molecule imaging techniques, the coupling between chemical processes (ATP hydrolysis) and mechanical processes (myosin force generation) has been directly determined. In this chapter, we describe these two manipulation techniques, especially microneedle method, in detail.

Original languageEnglish
Title of host publicationSingle Molecule Enzymology
Subtitle of host publicationMethods and Protocols
EditorsGregory I. Mashanov, Christopher Batters
Pages143-159
Number of pages17
DOIs
Publication statusPublished - 2011 Oct 24

Publication series

NameMethods in Molecular Biology
Volume778
ISSN (Print)1064-3745

Keywords

  • Laser trap
  • Mechanochemical -coupling
  • Microneedle
  • Myosin
  • Single-molecule imaging
  • Single-molecule manipulation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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