Single-molecule force microscopy of circularly permuted green fluorescent protein

Tong Wang; Ken Nakajima; Takako Kogure; Shinobu Yokokawa; Atsushi Miyawaki; Masahiko Kara

doi:10.1143/JJAP.43.5520

Single-molecule force microscopy of circularly permuted green fluorescent protein

Tong Wang, Ken Nakajima, Takako Kogure, Shinobu Yokokawa, Atsushi Miyawaki, Masahiko Kara

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We introduced new termini on surface loops of green fluorescent protein (GFP) by linking the original ones. The mechanical properties of two circularly permuted GFPs (cpGFPs) were examined by atomic force microscopy (AFM) and compared with those of the base GFPs. The unfolding results revealed different levels of the reduced mechanical stability of cpGFPs, and these levels were related to the proximity of the newly introduced termini to the central 'β-can'.

Original language	English
Pages (from-to)	5520-5523
Number of pages	4
Journal	Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume	43
Issue number	8 A
DOIs	https://doi.org/10.1143/JJAP.43.5520
Publication status	Published - 2004 Aug
Externally published	Yes

Keywords

'β-can'
Atomic force microscopy
Circular permutation
Force-extension curve
Green fluorescent protein

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

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10.1143/JJAP.43.5520

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Single-molecule force microscopy of circularly permuted green fluorescent protein. / Wang, Tong; Nakajima, Ken; Kogure, Takako; Yokokawa, Shinobu; Miyawaki, Atsushi; Kara, Masahiko.

In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 43, No. 8 A, 08.2004, p. 5520-5523.

Research output: Contribution to journal › Article › peer-review

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AU - Miyawaki, Atsushi

AU - Kara, Masahiko

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AB - We introduced new termini on surface loops of green fluorescent protein (GFP) by linking the original ones. The mechanical properties of two circularly permuted GFPs (cpGFPs) were examined by atomic force microscopy (AFM) and compared with those of the base GFPs. The unfolding results revealed different levels of the reduced mechanical stability of cpGFPs, and these levels were related to the proximity of the newly introduced termini to the central 'β-can'.

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KW - Force-extension curve

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Single-molecule force microscopy of circularly permuted green fluorescent protein

Abstract

Keywords

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