Single-molecule force microscopy of circularly permuted green fluorescent protein

Tong Wang, Ken Nakajima, Takako Kogure, Shinobu Yokokawa, Atsushi Miyawaki, Masahiko Kara

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

We introduced new termini on surface loops of green fluorescent protein (GFP) by linking the original ones. The mechanical properties of two circularly permuted GFPs (cpGFPs) were examined by atomic force microscopy (AFM) and compared with those of the base GFPs. The unfolding results revealed different levels of the reduced mechanical stability of cpGFPs, and these levels were related to the proximity of the newly introduced termini to the central 'β-can'.

Original languageEnglish
Pages (from-to)5520-5523
Number of pages4
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume43
Issue number8 A
DOIs
Publication statusPublished - 2004 Aug
Externally publishedYes

Keywords

  • 'β-can'
  • Atomic force microscopy
  • Circular permutation
  • Force-extension curve
  • Green fluorescent protein

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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