Single-molecule force microscopy of circularly permuted green fluorescent protein

Tong Wang, Ken Nakajima, Takako Kogure, Shinobu Yokokawa, Atsushi Miyawaki, Masahiko Kara

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    We introduced new termini on surface loops of green fluorescent protein (GFP) by linking the original ones. The mechanical properties of two circularly permuted GFPs (cpGFPs) were examined by atomic force microscopy (AFM) and compared with those of the base GFPs. The unfolding results revealed different levels of the reduced mechanical stability of cpGFPs, and these levels were related to the proximity of the newly introduced termini to the central 'β-can'.

    Original languageEnglish
    Pages (from-to)5520-5523
    Number of pages4
    JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
    Volume43
    Issue number8 A
    DOIs
    Publication statusPublished - 2004 Aug 1

    Keywords

    • 'β-can'
    • Atomic force microscopy
    • Circular permutation
    • Force-extension curve
    • Green fluorescent protein

    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

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