Single molecule detection from a large-scale SERS-active Au 79Ag 21 substrate

Hongwen Liu, Ling Zhang, Xingyou Lang, Yoshinori Yamaguchi, Hiroshi Iwasaki, Yasushi Inouye, Qikun Xue, Mingwei Chen

    Research output: Contribution to journalArticlepeer-review

    175 Citations (Scopus)


    Detecting and identifying single molecules are the ultimate goal of analytic sensitivity. Single molecule detection by surface-enhanced Raman scattering (SM-SERS) depends predominantly on SERS-active metal substrates that are usually colloidal silver fractal clusters. However, the high chemical reactivity of silver and the low reproducibility of its complicated synthesis with fractal clusters have been serious obstacles to practical applications of SERS, particularly for probing single biomolecules in extensive physiological environments. Here we report a large-scale, free standing and chemically stable SERS substrate for both resonant and nonresonant single molecule detection. Our robust substrate is made from wrinkled nanoporous Au 79Ag 21 films that contain a high number of electromagnetic "hot spots"with a local SERS enhancement larger than 10 9. This biocompatible gold-based SERS substrate with superior reproducibility, excellent chemical stability and facile synthesis promises to be an ideal candidate for a wide range of applications in life science and environment protection.

    Original languageEnglish
    Article number112
    JournalScientific reports
    Publication statusPublished - 2011

    ASJC Scopus subject areas

    • General


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